Saliva protein adsorption to the materials for prosthodontics and hydroxyapatite

口腔修复材料和羟基磷灰石对唾液蛋白的吸附

• 批准号: 17592045
• 负责人: MIYAKE Nahoko
• 金额: $ 2.11万
• 依托单位: Tokyo Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592045/
• 关键词: Zeta potential; Saliva protein adsorption; Materials for the prosthodontics; Hydroxyapatite; Laser irradiation; 接触角; レーザー

The aim of the present study was to clarify the correlation among saliva protein adsorption to the material for prosthodontics and hydroxyapatite and the surface zeta potential of those materials.Electrophoretic mobility was measured by an electrophoretic light-scattering spectrophotometer (ELS-800,Otsuka Electronics, Japan), using a capillary slablike sample cell that is particularly suitable for investigating the structure of a single microcapsule. The contact angle of sessile drop of distilled water was measured. The structure of the laser irradiated hydroxyapatite surfaces were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Adsorption experiments were performed in slablike sample cell in which 10mM NaCl were add to 2.0×10^<-5> mol BSA for 30min, then electrophoretic mobility was measured. A diode laser (LD15,Dentek Laser Systems Production, Austria) were irradiate HA plate (6.1Watt,60sec,300J/cm^2).The zeta potential of crown reisn was from -18.5mV±1.3 to 3.5mV±0.3. The zeta potential of the irradiation HA surfaces (-11.2mV±1.7) were grater tendency of the control surfaces (-9.1mV±1.9). The Laser irradiation inconsiderable make HA surfaces more hydrophobic. SEM observations showed that the irradiation HA surfaces were the same as the controls. AFM observations demonstrated that the irradiation HA surfaces were gradually melted and rounded. After the adsorption of BSA, the zeta potential of the irradiation HA surfaces (-23.7mV±8.1) and the control surfaces (-24.1mV±4.3) were increased.The present study indicates that crown resin might have possibility to control the adsorption saliva protein.

本研究的目的是阐明唾液蛋白在口腔修复材料和羟基磷灰石上的吸附与这些材料的表面Zeta电位之间的关系。使用一种特别适合于研究单个微囊结构的毛细管状样品池，使用电泳光散射分光光度计(ELS-800，大冢电子，日本)测量凝胶迁移率。测定了蒸馏水固着液滴的接触角。用扫描电子显微镜(SEM)和原子力显微镜(AFM)观察了激光辐照后羟基磷灰石表面的结构。将10 mM的氯化钠加入2.0×10~(-5)~(-5)摩尔牛血清白蛋白中30min，在黑板式样品池中进行吸附实验，然后测定其电泳迁移率。用半导体激光器(LD15，奥地利DenTek激光系统公司生产)照射HA板(6.1W，60秒，300J/cm~2)，冠状REISN的Zeta电位从-18.5 mV±1.3到3.5 mV±0.3。辐照后HA表面的Zeta电位(-11.2 mV±1.7)比对照表面(-9.1 mV±1.9)有更大的趋势。激光照射过多使HA表面疏水性增强。扫描电子显微镜观察表明，辐照后的羟基磷灰石表面与对照相同。AFM观察表明，辐照后的羟基磷灰石表面逐渐熔化、变圆。经BSA吸附后，辐照HA表面的Zeta电位(-23.7 mV±8.1)和对照表面的Zeta电位(-24.1 mV±4.3)均增加，提示冠状树脂可能具有控制唾液蛋白吸附的可能性。