Saliva protein adsorption to the materials for prosthodontics and hydroxyapatite

本研究的目的是阐明唾液蛋白对修复材料和羟基磷灰石的吸附及其表面zeta电位的关系。电泳迁移率由电泳光散射分光光度计（ELS-800，大冢电子公司，日本）测量，使用毛细管板样样品池，特别适合研究单个微胶囊的结构。测定了液滴在蒸馏水中的接触角。采用扫描电镜（SEM）和原子力显微镜（AFM）观察了激光照射羟基磷灰石表面的结构。吸附实验在板状样品池中进行，在2.0×10^<-5> mol BSA中加入10mM NaCl 30min，然后测定电泳迁移率。用二极管激光器（LD15,Dentek laser Systems Production, Austria）照射HA板（6.1瓦，60秒，300J/cm^2）。冠状树脂的zeta电位范围为-18.5mV±1.3 ~ 3.5mV±0.3。辐照表面（-11.2mV±1.7）的zeta电位高于对照表面（-9.1mV±1.9）的zeta电位。激光照射可使透明质酸表面更疏水。扫描电镜观察表明，辐照后的HA表面与对照组相同。原子力显微镜观察表明，辐照后的HA表面逐渐熔化并变圆。吸附BSA后，辐照HA表面（-23.7mV±8.1）和对照表面（-24.1mV±4.3）的zeta电位均升高。本研究表明，冠状树脂可能具有控制唾液蛋白吸附的可能性。

The aim of the present study was to clarify the correlation among saliva protein adsorption to the material for prosthodontics and hydroxyapatite and the surface zeta potential of those materials.Electrophoretic mobility was measured by an electrophoretic light-scattering spectrophotometer (ELS-800,Otsuka Electronics, Japan), using a capillary slablike sample cell that is particularly suitable for investigating the structure of a single microcapsule. The contact angle of sessile drop of distilled water was measured. The structure of the laser irradiated hydroxyapatite surfaces were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Adsorption experiments were performed in slablike sample cell in which 10mM NaCl were add to 2.0×10^<-5> mol BSA for 30min, then electrophoretic mobility was measured. A diode laser (LD15,Dentek Laser Systems Production, Austria) were irradiate HA plate (6.1Watt,60sec,300J/cm^2).The zeta potential of crown reisn was from -18.5mV±1.3 to 3.5mV±0.3. The zeta potential of the irradiation HA surfaces (-11.2mV±1.7) were grater tendency of the control surfaces (-9.1mV±1.9). The Laser irradiation inconsiderable make HA surfaces more hydrophobic. SEM observations showed that the irradiation HA surfaces were the same as the controls. AFM observations demonstrated that the irradiation HA surfaces were gradually melted and rounded. After the adsorption of BSA, the zeta potential of the irradiation HA surfaces (-23.7mV±8.1) and the control surfaces (-24.1mV±4.3) were increased.The present study indicates that crown resin might have possibility to control the adsorption saliva protein.