Saliva protein adsorption to the materials for prosthodontics and hydroxyapatite

本研究的目的是澄清唾液蛋白质吸附到材料的修复和羟基磷灰石和这些材料的表面zeta电位之间的相关性，电泳迁移率通过电泳光散射分光光度计（ELS-800，大冢电子，日本），使用毛细管板状样品池，特别适合于调查一个单一的微胶囊的结构。测定了蒸馏水静滴的接触角。利用扫描电子显微镜（SEM）和原子力显微镜（AFM）观察了激光辐照后羟基磷灰石表面的结构。在板状样品池中，加入10 mM NaCl，在2.0×10 ~（-1）<-5>mol BSA中吸附30 min，然后测定电泳迁移率。半导体激光器（LD 15，Dentek Laser Systems Production，Austria）以6.1W、60秒、300 J/cm ^2的能量照射HA板，冠状树脂的zeta电位为-18.5mV ~1.3 ~ 3.5mV±0.3。辐照HA表面的zeta电位（-11.2mV &lt;$1.7）比对照表面（-9.1mV &lt;$1.9）有更大的趋势。激光照射使HA表面疏水性增强。扫描电镜观察表明，辐照后的HA表面与对照组相同。原子力显微镜观察表明，辐照后的HA表面逐渐熔化并变圆。吸附BSA后，辐照HA表面（-23.7mV &lt;$8.1）和对照表面（-24.1mV &lt;$4.3）的zeta电位均有所升高，表明冠醚树脂有可能控制唾液蛋白的吸附。

The aim of the present study was to clarify the correlation among saliva protein adsorption to the material for prosthodontics and hydroxyapatite and the surface zeta potential of those materials.Electrophoretic mobility was measured by an electrophoretic light-scattering spectrophotometer (ELS-800,Otsuka Electronics, Japan), using a capillary slablike sample cell that is particularly suitable for investigating the structure of a single microcapsule. The contact angle of sessile drop of distilled water was measured. The structure of the laser irradiated hydroxyapatite surfaces were observed by scanning electron microscopy (SEM) and atomic force microscopy (AFM). Adsorption experiments were performed in slablike sample cell in which 10mM NaCl were add to 2.0×10^<-5> mol BSA for 30min, then electrophoretic mobility was measured. A diode laser (LD15,Dentek Laser Systems Production, Austria) were irradiate HA plate (6.1Watt,60sec,300J/cm^2).The zeta potential of crown reisn was from -18.5mV±1.3 to 3.5mV±0.3. The zeta potential of the irradiation HA surfaces (-11.2mV±1.7) were grater tendency of the control surfaces (-9.1mV±1.9). The Laser irradiation inconsiderable make HA surfaces more hydrophobic. SEM observations showed that the irradiation HA surfaces were the same as the controls. AFM observations demonstrated that the irradiation HA surfaces were gradually melted and rounded. After the adsorption of BSA, the zeta potential of the irradiation HA surfaces (-23.7mV±8.1) and the control surfaces (-24.1mV±4.3) were increased.The present study indicates that crown resin might have possibility to control the adsorption saliva protein.