Detection of Potential Biomarkers of Lymph Node Metastasis in Oral Cancer by HOX Gene Expression Profiles

通过 HOX 基因表达谱检测口腔癌淋巴结转移的潜在生物标志物

基本信息

批准号：
17592060
负责人：
KASHIWAZAKI Haruhiko
金额：
$ 2.3万
依托单位：
HOKKAIDO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17592060/
关键词：
HOX Gene Oral Cancer Lymph Node Metastasis

项目摘要

Background.HOX genes encode transcription factors that function as master regulators in embryogenesis. Human HOX genes consist of 39 genes arranged in four clusters (HOXA, B, C and D) localized on chromosome 7,17,12 and 2, respectively. We hypothesized that the dysregulated expression of HOX genes was associated with tumor development and malignant progression.Methods.In this study, the expression levels of39 HOX genes in 31 human oral squamous cell carcinoma (OSCC), 11 dysplasia and 10normal mucosa tissues were quantified by a comprehensive analysis system based on the real-time RT-PCR method.Results.We found that the expression levels of 18 HOX genes (HOXA1,A2,A3,A5,A9,B3,B6,B7,B9,C4,C6,C8,C9,C11,C13,D9,D10 andD11) in the OSCC tissues were significantly higher than those in the normal mucosa tissues.The dysplasia tissues showed significantly high expression of HOXA2,A3,B3 and D10 compared to normal mucosa tissues whereas they showed significantly low expression of HOXA1,B7,B9 and C8 compared to OSCC. The OSCC with lymph node metastasis showed high expression of HOXC6 compared to the OSCC without it. Cluster analysis of all the normal mucosa, dysplasia and OSCC tissues according to 39 HOX gene expression levels classified them into two groups, a normal mucosa group and a OSCC group, except one normal mucosa ; and the dysplasia tissues were classified into either a normal mucosa group or a OSCC group.Conclusions.These results indicate that aberrant expressions of particular HOX genes are implicated in the development of oral dysplasia and OSCC and these expression profiles could provide a biomarker system for predicting the risk of lymph node metastasis in OSCC.

背景。HOX基因编码转录因子，这些因子在胚胎发生中充当主调节剂。人HOX基因分别由39个基因组成，分别以四个簇（Hoxa，b，c和d）排列在7,17,12和2染色体上。我们假设HOX基因的表达失调与肿瘤的发展和恶性进展有关。方法。在这项研究中，31个人口腔鳞状细胞癌（OSCC）中39 HOX基因的表达水平，11个发育不良和11个正态粘膜组织的综合化度是基于18个整体的整体，基于18个整体的整体，该方法是基于18个植物的概念。（HOXA1，A2，A3，A3，A5，B3，B3，B6，B7，B9，C4，C4，C6，C8，C9，C9，C9，C11，C11，C13，D9，D9，D10和D10）在OSCC组织中显着高于正常粘膜组织中的粘液组织中的粘液症组织较高的粘液组织。与OSCC相比，它们显示出HOXA1，B7，B9和C8的表达明显低。与没有IT的OSCC相比，具有淋巴结转移的OSCC显示出HOXC6的高表达。根据39个HOX基因表达水平分为两组，对所有正常粘膜，发育不良和OSCC组织的聚类分析，除非正常粘膜除外；并将其发育不良组织分为正常的粘膜组或OSCC组。结论。这些结果表明，这些特定HOX基因的异常表达与口服异常增生和OSCC的发展有关，这些表达曲线可以为预测OSCC淋巴结质体的风险提供生物标记系统。