Exploring cellular changes during the production of an extracellular sunscreen pigment found in terrestrial green algae (Zygnematophyceae, Streptophyta)

探索陆地绿藻（Zygnematophyceae、Streptophyta）中发现的细胞外防晒色素生产过程中的细胞变化

• 批准号: 491244984
• 负责人: Professor Dr. Sebastian Hess
• 金额: --
• 依托单位: Arbeitsgruppe Molekulare Pflanzenphysiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/491244984?language=en
• 关键词: Exploring; cellular; changes; during; production

The ‘conjugating green algae’ (Zygnematophyceae) are the closest relatives of land plants and, hence, highly interesting from an evolutionary perspective. Given the immense structural and ecological diversity of these algae, their adaptations to the various habitats are still poorly understood. In a new genus of unicellular, terrestrial zygnematophytes (Serritaenia) we, recently, discovered an extracellular sunscreen pigment that can be experimentally induced by ultraviolet radiation. In the proposed project we will explore the cellular changes during the production of this yet unknown compound with comparative transcriptomics, metabolomics as well as electron microscopy. These techniques will enable us to (1) identify metabolic pathways involved in the production of Serritaenia’s sunscreen, (2) determine the compound itself (or fragments), (3) shed light on the molecular components involved in UV perception, and (4) gain deeper insights into the secretion of pigmented mucilage in Serritaenia. With this, we will uncover the cellular and molecular basis of a unique photoprotective strategy in the zygnematophytes, and enable exciting comparisons with algal and plant model systems.

“接合绿色藻类”（接合藻纲）是陆地植物的近亲，因此从进化的角度来看非常有趣。鉴于这些藻类的巨大结构和生态多样性，它们对各种栖息地的适应仍然知之甚少。在一个新属的单细胞，陆生zygnematophytes（Serritaenia），我们最近，发现了一种细胞外防晒色素，可以通过实验诱导的紫外线辐射。在拟议的项目中，我们将探索细胞的变化，在生产这种未知的化合物与比较转录组学，代谢组学以及电子显微镜。这些技术将使我们能够（1）确定参与生产Serritaenia防晒霜的代谢途径，（2）确定化合物本身（或片段），（3）阐明参与UV感知的分子组分，（4）更深入地了解Serritaenia中色素粘液的分泌。有了这个，我们将揭示一个独特的光保护策略在zynematophytes的细胞和分子基础，并使令人兴奋的比较与藻类和植物模型系统。