Exploring the impact of Xanthomonas euvesicatoria effector XopL on ABA signaling and host cell metabolism.

探索 Xanthomonas euvesicatoria 效应子 XopL 对 ABA 信号传导和宿主细胞代谢的影响。

• 批准号: 492635023
• 负责人: Dr. Jessica Erickson, Ph.D.
• 金额: --
• 依托单位: Abteilung Biochemie pflanzlicher Interaktionen
• 依托单位国家: 德国
• 项目类别: WBP Position
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 2023-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/492635023?language=en
• 关键词: Exploring; impact; Xanthomonas; euvesicatoria; effector

Xanthomonads are a genus of Gram-negative phytopathogens that cause disease in hundreds of economically important crop species worldwide. Xanthomonads are hemibiotrophic pathogens which spend a disproportionate amount of their life cycle living in/feeding off living plant tissue (biotrophic phase), but ultimately cause cell death during later stages of the infection (necrotrophic phase). Biotrophic growth phases require constant subversion of the plant immune system, and simultaneous conversion of the plant cell apoplast into a habitable environment. To address these challenges Xanthomonads utilize the type-III secretion system (T3SS) to inject bacterial-derived type III-secreted effector proteins (T3Es) directly into the host cytosol. T3Es are essential to pathogen virulence and it is generally understood that once inside, T3Es manipulate plant cell processes to support life in the apoplast. Intensive research into effector targets have revealed many of the plant processes that are vulnerable to pathogen attack, but exactly how these effector functions translate to changes in the apoplast where Xanthomonas actually resides are generally understudied. Going forward an understanding of Xanthomonas virulence requires that we link T3E function inside the cell to outcomes for the bacteria in the intracellular space.X. euvesicatoria (Xe) strain 85-10, the causal agent of bacterial spot disease on pepper and tomato, secretes 36 effector proteins, many of which are termed Xanthomonas outer proteins (Xops). One such protein, XopL, displays E3 ligase function in plants, where it hijacks the plant ubiquitylation cascade to modify plant substrate proteins. Preliminary work on XopL suggests that it might manipulate host cell metabolism by targeting a known ABA signaling component, ARIA (ARM REPEAT PROTEIN INTERACTING WITH ABF), for degradation. ARIA regulates the expression of genes important for the production of primary and secondary metabolites known to influence the success of pathogen growth in the apoplast. The goal of the proposed project is to utilize the XopL-ARIA interaction as a case study to determine whether the manipulation of the ABA signal cascade inside the cell can be linked to changes in the apoplast.

黄单胞菌属是一种革兰氏阴性植物病原体，其在全世界数百种经济上重要的作物物种中引起疾病。黄单胞菌是半活体营养型病原体，其在其生命周期中花费不成比例的量生活在活植物组织中/以活植物组织为食（活体营养期），但最终在感染的后期阶段（坏死营养期）引起细胞死亡。生物营养生长阶段需要不断颠覆植物免疫系统，并同时将植物细胞质外体转化为可居住的环境。为了解决这些挑战，黄单胞菌利用III型分泌系统（T3 SS）将细菌来源的III型分泌的效应蛋白（T3 E）直接注射到宿主细胞质中。T3 E对于病原体毒力是必不可少的，并且通常理解，一旦进入，T3 E操纵植物细胞过程以支持质外体中的生命。深入研究效应靶标已经揭示了许多易受病原体攻击的植物过程，但这些效应功能如何转化为黄单胞菌实际居住的质外体中的变化，通常研究不足。为了进一步了解黄单胞菌的毒力，我们需要将细胞内的T3 E功能与细胞内空间中细菌的结果联系起来。辣椒和番茄细菌性斑点病的致病菌--一种这样的蛋白质XopL在植物中显示E3连接酶功能，其中它劫持植物泛素化级联以修饰植物底物蛋白。对XopL的初步研究表明，它可能通过靶向已知的阿坝信号组分ARIA（与ABF相互作用的臂重复蛋白）降解来操纵宿主细胞代谢。ARIA调节对已知影响质外体中病原体生长成功的初级和次级代谢产物的产生重要的基因的表达。拟议项目的目标是利用XopL-ARIA相互作用作为案例研究，以确定细胞内阿坝信号级联的操纵是否与质外体的变化有关。