Postsynaptic receptor plasticity and transsynaptic communication in storage of memory components in the mushroom bodies

蘑菇体内存储记忆成分的突触后受体可塑性和跨突触通讯

• 批准号: 495851732
• 负责人: Professor Dr. David Owald
• 金额: --
• 依托单位: Institut für Neurophysiologie (CCM)
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/495851732?language=en
• 关键词: Postsynaptic; receptor; plasticity; transsynaptic; communication

Memories typically represent multifaceted experiences, with individual components likely represented as parallel traces. In Drosophila, it is well established that, in general, differential olfactory conditioning paradigms give rise to memories stored as synaptic changes at the level of the mushroom bodies (MBs). While it is conceivable that differential conditioning will give rise to individual separate memories, and consequently molecular memory traces representing the presence of a reward or a punishment or the absence of either, how and where these individual facets are stored remains unclear. This proposal builds on (1) our previous work identifying two distinct MB output compartments involved in either appetitive or aversive memory storage and (2) our recent discovery of postsynaptic plasticity that exists next to presynaptic mechanisms in the context of appetitive memories. We will address the hypothesis that each MB output compartment can store both, an aversive and an appetitive trace, by utilizing the postsynaptic alongside the presynaptic compartment, to encompass information for instance representing the presence and the absence of a reward. Using absolute conditioning in combination with in vivo functional and receptor imaging protocols that we developed over the last years, we will address mechanisms of postsynaptic receptor plasticity in the context of aversive and appetitive memories. We will further investigate potential transsynaptic interplay during memory formation by targeted intervention of postsynaptic receptors or the presynaptic protein composite.

记忆通常代表多方面的经历，各个组成部分可能表现为平行的痕迹。在果蝇中，它是公认的，在一般情况下，不同的嗅觉条件反射范式引起的记忆存储在蘑菇体（MB）的水平上的突触变化。虽然可以想象，不同的条件作用会产生个体独立的记忆，从而产生代表奖励或惩罚存在或不存在的分子记忆痕迹，但这些个体方面是如何以及在哪里存储的仍然不清楚。这个提议建立在（1）我们以前的工作，确定两个不同的MB输出隔间参与食欲或厌恶的记忆存储和（2）我们最近发现的突触后可塑性，存在旁边的突触前机制在食欲记忆的背景下。我们将解决的假设，每个MB输出隔室可以存储，一个厌恶和一个食欲的痕迹，通过利用突触后旁边的突触前隔室，包括信息，例如代表的存在和不存在的奖励。使用绝对条件反射结合在体内功能和受体成像协议，我们在过去几年中开发的，我们将解决在厌恶和食欲记忆的背景下，突触后受体可塑性的机制。我们将通过有针对性的干预突触后受体或突触前蛋白复合物，进一步研究记忆形成过程中潜在的跨突触相互作用。