Molecular Analyses of Mechanisms of Pollination and Ferulzation

(1)甘蓝型油菜的自交不亲和性：利用多种生化和分子生物学方法对甘蓝型油菜花粉提取液和花粉基因进行了分析，发现了花粉S决定子的候选分子。进一步对基因组克隆进行了分析，包括SLG和SRK。并发现了几个他会在花粉和花药中特别表达的基因。(2)日本梨及其近缘植物的自交不亲和性我们克隆了日本梨的S-核糖核酸酶基因，并成功地获得了S-核糖核酸酶的晶体，用于X射线分析以确定S-核糖核酸酶的分子结构。(3)油菜的自交不亲和性。将该基因定位在距S基因座1.2cM的位置。(4)胚性发生利用突变体植株，我们已鉴定出12个影响水稻早期胚胎发生的基因座。我们分析了一个在胡萝卜早期胚胎和不定胚中表达的基因，并与拟南芥的一个同源基因进行了比较，在启动子区域确定了一个顺式序列。

(1) Self-incompatibility of Brassica.We have analyzed pollen extract and pollen mRNA of Brassica campestris using various biochemical and molecular biological methods and found candidate molecules of the pollen S-determinant. Further we analyzed the genomic clone including SLG and SRK. and found several genes which would he expressed especially in pollen and anther. We have succeeded in visualization of SRK and SLO genes in the DNA fibets of Brassica rapa with FISH.(2) Self-incompatibility of Japanese pear and related plantsWe have cloned and sequenced S-RNase genes of Japanese pear and further we have succeeded in obtaing crystals of S-Rnase for X-ray analysis to determine the molecular structure of S-RNase.(3) Self-incompanbility of IpomeaWe have isolated a cDNA encoding a stigma-specific protein from wild species of Ipomea and analyzed its expression in the stigma. The gene was mapped at the distance of 1.2 cM from the S-locus.(4) EmbriogenesisWe have identified 12 loci affecting embryogenesis of rice at early stages using mutant plants. We have analyzed a gene expressed in embryo and adventitious embryo of carrot at a early stage and identified a cis sequence at the promoter region in comparison of a homologous gene of Arabidopsis.