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Study on the genomic imprinting system and plasticity of the male germ cell genome

雄性生殖细胞基因组印记系统及可塑性研究

基本信息

批准号：
11234206
负责人：
OGURA Atsuo
金额：
$ 19.58万
依托单位：
The Institute of Physical and Chemical Research (RIKEN) (2002)National Institute of Infectious Diseases (1999-2001)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research on Priority Areas
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2002
项目状态：
已结题

项目摘要

In this study, we examined embryos, fetuses, and animals produced by microinsemination or nuclear transfer techniques using male germ cells to know their genetic and epigenetic changes, which play the most integral part of in the acquisition of the properties of male gametes. Expression analysis of imprinted genes in fetuses reconstructed from primordial germ cells at 11.5 dpc and 12.5 dpc demonstrated that erasure of the parental imprinting memory occurred at 11.5 dpc and has completed by 12.5 dpc. Analysis of fetuses derived from gonocytes at day 3.5 after birth indicated that the paternal imprinting has not yet established by this stage. The first wave round spermatids at 17 days of age participated in normal development into offspring, indicating that the paternal imprinting has completed before meiosis of the first wave of spermatogenesis. The chromosomes of primary spermatocytes, spermatogenic cells before meiosis, underwent normal meiotic divisions after introduced into MI oocytes, and subsequently supported full term development. Detailed gene expression analysis demonstrated that each imprinted gene has each own schedule of erasure and establishment of imprinting marks. The developmental ability of embryos reconstructed from primordial germ cells at 11.5 dpc suggested that the totipotency of the male germ cell genome is probably maintained until 11.5 dpc or a little earlier stage.

在这项研究中，我们检查了胚胎，胎儿和使用雄性生殖细胞通过显微授精或核移植技术产生的动物，以了解它们的遗传和表观遗传变化，这些变化在获得雄性配子的特性中起着最重要的作用。印迹基因在胚胎发育11.5 dpc和12.5 dpc的胚胎中的表达分析表明，父母印迹记忆的消除发生在11.5 dpc，并已完成12.5 dpc。对出生后3.5天来自生殖细胞的胎儿的分析表明，该阶段尚未建立父系印记。17日龄的第一波圆形精子细胞参与了正常的子代发育，表明在第一波精子发生的减数分裂之前，父本印记已经完成。初级精母细胞（减数分裂前的生精细胞）的染色体导入MI卵母细胞后，进行正常的减数分裂，并随后支持足月发育。详细的基因表达分析表明，每个印迹基因有自己的时间表删除和建立印迹标记。原始生殖细胞重构胚在11.5dpc时的发育能力表明，雄性生殖细胞基因组的全能性可能维持到11.5dpc或稍早的阶段。