Investigation on high throughput chemical synthesis by utilizing micro chemical systems

本研究的目的是构建高通量合成微系统，包括评价合成效率和建立微系统设计指南。研究内容包括：（1）高通量合成微系统的构建;（2）堆积式生产系统的开发;（3）微型组合合成系统的开发;（4）利用微空间的特点建立微型反应场。首先，我们提取了微空间中化学反应的特征，并结合微单元操作（MUO）建立了我们独创的微芯片连续流化学处理（CFCP）方法。然后，我们构建了微型化学合成系统、堆积反应器系统和组合合成系统。我们还通过开发新的MUO来扩大我们的方法。实现了微逆流萃取、液-液或气-液多相流的稳定化、细胞的长期培养、微生物反应器的基础技术等。此外，反应器的尺寸被推导到100 nm的规模和连接的微和纳米系统建立。

Objective of this research was construction of high throughput synthesis microsystem, which included evaluation of synthesis efficiency and establishment of guidelines for the microsystem design. The following issues were investigated : (1)Construction of highthroughput synthesis microsystem, (2)Development of piled-up production system, (3)Development of micro combinatorial synthesis system, and (4)Creation of micro reaction field utilizing features of microspace. First of all, we extracted characteristics of chemical reactions in microspace and established our original methodology of continous flow chemical processing (CFCP) on microchip by combining micro unit operations (MUO). Then, we constructed micro chemical synthesis system, piled-up reactor system, and combinatorial synthesis system. We also widen our methodology by developing novel MUOs. For example, micro counter-current extraction, stabilization of liquid-liquid or gas-liquid multiphase flows, long-term cultivation of cells, and fundamental technologies for micro bio-reactor were realized. Furthermore, size of reactor was deduced to a 100-nm scale and connection of micro-and nano-systems was established.