Three-dimensional structure analysis of bacterial pheromone receptor and transporter membrane protein and its application to the design of novel bacteriostatic agents

粪肠球菌明胶酶的产生受环肽信息素GBAP的调节。我们确定了GBAP的三级结构，并确定了其信息素活性的重要残基。我们还根据结构信息通过Ala突变开发了GBAP拮抗剂。我们在大肠杆菌中表达，纯化和结晶重组Fsrc，GBAP受体膜蛋白。FsrC-GBAP复合物的晶体衍射X射线至5.5-A分辨率。

The gelatinase production by Enterococcus faecalis is regulated by the cyclic peptide pheromone, GBAP. We determined the tertiary structure of GBAP and identified the important residues for its pheromone activity. We also developed GBAP antagonists by Ala mutations based on the structural information. We expressed in Escherichia coli, purified and crystallized recombinant FsrC, the GBAP receptor membrane protein. A crystal of FsrC-GBAP complex diffracted X-rays to 5.5-Aresolution.