Integrated cancer research using in vivo models

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We organized 7 groups to support cancer research in Japan during 2005 and 2009 academic year. We produced 1463 transgenic or knockout mouse strains and responded to 191 consultations by distributing materials such as targeting vectors. The number of request for conditional gene targeting instead of simple knockout is increasing in recent years. Microbiological monitoring and genetic monitoring using biochemical/microsattelite markers were accomplished for 25,769 samples in 1798 cases and 1,367 samples, respectively. Due to these efforts, infection to laboratory mouse is rarely seen under SPF condition in these years. We cryopreserved embryos and sperms for 7,985 strains and supplied 4,358 strains. Infected 2739 strains were recovered and cleaned from viruses using in vitro fertilization technique. We started to supply embryos under refrigerated condition using a new method by which recovered embryo can be preserved for 3 or 4 days. We held the course for reproductive engineering for 24 times to 136 trainees. CARD-RBASE was established with necessary function such as links to other web sites. We supplied 7,357 cell lines for cancer researchers. We also carried out pathologic analyses for 55 genetically engineered mouse strains upon request.