Integrated cancer research using in vivo models

在2005 - 2009学年期间，我们组织了7个小组来支持日本的癌症研究。我们生产了1463个转基因或基因敲除小鼠品系，并通过分发靶向载体等材料回应了191次咨询。近年来，要求用条件基因打靶代替简单基因敲除的呼声越来越高。利用生物化学/微卫星标记，分别对1798个病例中的25，769个样本和1，367个样本进行了微生物监测和遗传监测。由于这些努力，近年来在SPF条件下很少见到实验室小鼠感染。我们冷冻保存了7，985株胚胎和精子，并提供了4，358株。用体外受精技术对感染的2739株菌株进行了回收和病毒清除。我们开始使用一种新的方法在冷藏条件下提供胚胎，通过这种方法，回收的胚胎可以保存3或4天。举办生殖工程学培训班24期，培训学员136人。CARD-RBASE的建立具有必要的功能，例如与其他网站的链接。我们为癌症研究人员提供了7，357个细胞系。我们还应要求对55个基因工程小鼠品系进行了病理学分析。

We organized 7 groups to support cancer research in Japan during 2005 and 2009 academic year. We produced 1463 transgenic or knockout mouse strains and responded to 191 consultations by distributing materials such as targeting vectors. The number of request for conditional gene targeting instead of simple knockout is increasing in recent years. Microbiological monitoring and genetic monitoring using biochemical/microsattelite markers were accomplished for 25,769 samples in 1798 cases and 1,367 samples, respectively. Due to these efforts, infection to laboratory mouse is rarely seen under SPF condition in these years. We cryopreserved embryos and sperms for 7,985 strains and supplied 4,358 strains. Infected 2739 strains were recovered and cleaned from viruses using in vitro fertilization technique. We started to supply embryos under refrigerated condition using a new method by which recovered embryo can be preserved for 3 or 4 days. We held the course for reproductive engineering for 24 times to 136 trainees. CARD-RBASE was established with necessary function such as links to other web sites. We supplied 7,357 cell lines for cancer researchers. We also carried out pathologic analyses for 55 genetically engineered mouse strains upon request.