Integrated cancer research using in vivo models

使用体内模型的综合癌症研究

• 批准号: 17012017
• 负责人: YAMAMURA Kenichi
• 金额: $ 246.78万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17012017/
• 关键词: 遺伝子改変マウス; 相同組換え; 凍結保存; 微生物学的モニタリング; 遺伝学的モニタリング; データベース; 病理形態学; 組織診断; 遺伝子改変マスウ; トランスジェニック; ノックアウト; 遺伝子トラップ

We organized 7 groups to support cancer research in Japan during 2005 and 2009 academic year. We produced 1463 transgenic or knockout mouse strains and responded to 191 consultations by distributing materials such as targeting vectors. The number of request for conditional gene targeting instead of simple knockout is increasing in recent years. Microbiological monitoring and genetic monitoring using biochemical/microsattelite markers were accomplished for 25,769 samples in 1798 cases and 1,367 samples, respectively. Due to these efforts, infection to laboratory mouse is rarely seen under SPF condition in these years. We cryopreserved embryos and sperms for 7,985 strains and supplied 4,358 strains. Infected 2739 strains were recovered and cleaned from viruses using in vitro fertilization technique. We started to supply embryos under refrigerated condition using a new method by which recovered embryo can be preserved for 3 or 4 days. We held the course for reproductive engineering for 24 times to 136 trainees. CARD-RBASE was established with necessary function such as links to other web sites. We supplied 7,357 cell lines for cancer researchers. We also carried out pathologic analyses for 55 genetically engineered mouse strains upon request.

在2005-2009学年，我们组织了7个小组来支持日本的癌症研究。我们生产了1463个转基因或基因敲除小鼠品系，并通过分发靶向载体等材料回应了191个咨询。近年来，要求有条件的基因打靶而不是简单的基因敲除的数量正在增加。利用生化/微卫星标记分别对1798例25769份样本和1367份样本进行了微生物监测和遗传监测。由于这些努力，近年来在SPF条件下对实验鼠的感染很少见。我们冷冻保存了7985个菌株的胚胎和精子，提供了4358个菌株。用体外受精技术从病毒中回收和清除感染的2739株病毒。我们开始在冷藏条件下提供胚胎，采用一种新的方法，回收的胚胎可以保存3-4天。我们为136名学员举办了24次生殖工程课程。建立了Card-Rbase，具有必要的功能，如与其他网站的链接。我们为癌症研究人员提供了7357个细胞系。我们还应要求对55个转基因小鼠品系进行了病理分析。

项目成果

Positive regulation of steroidogenic acute regulatory protein (StAR)gene expression through the interaction between Dlx and GATA-4 for testicular steroidogenesis.

通过 Dlx 和 GATA-4 之间的相互作用对睾丸类固醇生成的类固醇生成急性调节蛋白 (StAR) 基因表达进行正向调节。

• 发表时间: 2008
• 期刊: Endocrinology 149
• 作者: 千田隆夫;尾之内高慶;高雄啓三;宮川剛;矢崎沙織;Nishida H.
• 通讯作者: Nishida H.

Isolation and identification procedure for Staphylococcus aureus in laboratory mice and rats by combined use of chromogenic X-SA agar and specific polvmerase chain reaction

X-SA显色琼脂联合特异性聚合酶链反应分离鉴定实验室小鼠和大鼠金黄色葡萄球菌

• 发表时间: 2009
• 期刊: J. Vet. Med. Sci. 71
• 作者: Hayashimoto;N.;et.al.
• 通讯作者: et.al.

Angiopoietin-related growth factor antagonizes obesity and insulin resistance

• DOI: 10.1038/nm1214
• 发表时间: 2005-04-01
• 期刊: NATURE MEDICINE
• 影响因子: 82.9
• 作者: Oike, Y;Akao, M;Suda, T
• 通讯作者: Suda, T

Inflammatory processes triggered by H. pylori infection cause aberrant DNA methylation in gastric epithelial cells.

幽门螺杆菌感染引发的炎症过程会导致胃上皮细胞异常 DNA 甲基化。

• 发表时间: 2010
• 期刊: Cancer Res 70
• 作者: Niwa;T.;et al.
• 通讯作者: et al.

IQGAP2 inactivation through aberrant methylation and promotion of invasion in gastric cancer cells.

IQGAP2 通过异常甲基化失活并促进胃癌细胞的侵袭。

• 发表时间: 2008
• 期刊: Int. J. Cancer 122
• 作者: Jin;S.;et. al.
• 通讯作者: et. al.