Genome-wide study on pathogenesis of bacteria causing nosocomial infection

引起医院感染的细菌发病机制的全基因组研究

• 批准号: 17019048
• 负责人: SUGAI Motoyuki
• 金额: $ 62.4万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research on Priority Areas
• 财政年份: 2005
• 资助国家: 日本
• 起止时间: 2005 至 2009
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17019048/
• 关键词: 黄色ブドウ球菌; 緑膿菌; セラチア; 病原性; マイクロアレイ; ゲノム; 院内感染

We employed Staphylococcus aureus, Serratia marcescens, Pseudomonas aeruginosa as target agent causing nosocomial infection. For S. aureus, we delineated gene sets present in S. aureus causing certain diseases and analyzed candidate genes to detect or foresee specific virulence of S. aureus. For S. marcescens, we screened for virulence-related genes by animal experiment and investigated for factors (genes) involved in human serum resistance. For P. aeruginosa, we investigated for factors (genes) involved in human serum resistance. We also analyzed characteristics and antimicrobial resistance mechanism of MDRP clones.

以金黄色葡萄球菌、粘质沙雷菌、铜绿假单胞菌为致医院感染的靶菌。对于金黄色葡萄球菌，我们描述了金黄色葡萄球菌中引起某些疾病的基因集，并分析了候选基因，以检测或预测金黄色葡萄球菌的特定毒力。针对粘质葡萄球菌，我们通过动物实验筛选了毒力相关基因，并研究了与人血清耐药相关的因素（基因）。对于铜绿假单胞菌，我们研究了参与人血清耐药的因素（基因）。我们还分析了MDRP克隆的特性和耐药机制。

项目成果

Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges

• DOI: 10.1074/jbc.m509691200
• 发表时间: 2006-01-06
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Lu, JZQ;Fujiwara, T;Sakon, J
• 通讯作者: Sakon, J

Classification of OprD sequence and correlation with antimicrobial activity of carbapenem agents in Pseudomonas aeruginosa clinical isolates collected in Japan

• DOI: 10.1111/j.1348-0421.2009.00137.x
• 发表时间: 2009-07-01
• 期刊: MICROBIOLOGY AND IMMUNOLOGY
• 影响因子: 2.6
• 作者: Sanbongi, Yumiko;Shimizu, Atsuyuki;Gotoh, Naomasa
• 通讯作者: Gotoh, Naomasa

Epidemiology, variable genetic organization and regulation of the EDIN-B toxin in Staphylococcus aureus from bacteremic patients

菌血症患者金黄色葡萄球菌中 EDIN-B 毒素的流行病学、可变遗传组织和调控

• 发表时间: 2010
• 期刊: Microbiology 78
• 作者: Gefron C.Franke;et al.
• 通讯作者: et al.

市中獲得緑膿菌における薬剤耐性機構の検討

社区获得性铜绿假单胞菌耐药机制的研究

• 发表时间: 2007
• 作者: 甲田俊太郎;小原勝;桑原正雄;碓井亞;菅井基行
• 通讯作者: 菅井基行

Molecular epidemiology of metallo-β-lactamase-producing Pseudomonas aeruginosa in Hiroshima, Japan

日本广岛产金属-β-内酰胺酶铜绿假单胞菌的分子流行病学

• 发表时间: 2007
• 作者: Shuntaro Koda;Masaru Ohara;Tsuguru Usui;Motoyuki Sugai
• 通讯作者: Motoyuki Sugai