Intranuclear action of Cell cycle-specific growth inhibitory factor, CDT

细胞周期特异性生长抑制因子 CDT 的核内作用

• 批准号: 20592140
• 负责人: SUGAI Motoyuki
• 金额: $ 3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2008
• 资助国家: 日本
• 起止时间: 2008 至 2010
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-20592140/
• 关键词: 口腔細菌; CDT; 細胞死; 細胞周期; 歯周病; Aggregatibactor actinomycetemcomitans; Aggregatibacter actinomycetemcomitans; periodontal diseases; oral; DNase; homologous recombination; apoptosis; Dnase; DNA; アポトーシス

When CdtB was expressed in yeat, Cdt acted as cytolethal and inhibited cell cycle at S/G2. Effect of mutation of DNA repair system on CDT-induced cell death was studied. Mutants of ead51, mre11, rad50 and wrs2 were found to be highly sensitive to CdtB. Further effect of mutation of apoptotic pathway on CDT-induced cell death was studied. Mutants of yca1 and aif1 did not change their susceptibility to CdtB. These resuts suggest that Cdt-induced yeast cell death is not dependent on apoptosis.

当CdtB在1年内表达时，Cdt具有细胞致死作用，并以S/G2水平抑制细胞周期。研究了DNA修复系统突变对cdt诱导的细胞死亡的影响。发现突变体ead51、mre11、rad50和wrs2对CdtB高度敏感。进一步研究凋亡通路突变对cdt诱导的细胞死亡的影响。突变体yca1和aif1对CdtB的易感性没有改变。这些结果表明cdt诱导的酵母细胞死亡不依赖于细胞凋亡。

项目成果

Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 and Jurkat cells.

细胞致死膨胀毒素可诱导 MOLT-4 和 Jurkat 细胞中依赖 caspase 和不依赖 caspase 的细胞死亡。

• 发表时间: 2008
• 期刊: Infection and Immunity 76(10)
• 作者: Ohara;M.;Hayashi;T.;Kusunoki;Y.;Nakachi;K.;Fujiwara;T.;Komatsuzawa;H.;Sugai;M.
• 通讯作者: M.

Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells

细胞致死膨胀毒素诱导 MOLT-4 细胞中 caspase 依赖性和非依赖性细胞死亡

• 发表时间: 2008
• 期刊: Infect.Immun. 76
• 作者: Ohara;M.
• 通讯作者: M.

Topical application of Aggregatibacter actinomycetemcomitans CDT induces cell cycle arrest in the rat gingival epithelium in vivo

局部应用放线菌聚集菌 CDT 诱导大鼠体内牙龈上皮细胞周期停滞

• 发表时间: 2011
• 期刊: Journal of Periodontal Research
• 影响因子: 3.5
• 作者: Ohara;M.;et al.
• 通讯作者: et al.

Cytolethal Distending Toxin Type I and Type IV Genes Are Framed with Lambdoid Prophage Genes in Extraintestinal Pathogenic Escherichia coli

• DOI: 10.1128/iai.00962-08
• 发表时间: 2009-01-01
• 期刊: INFECTION AND IMMUNITY
• 影响因子: 3.1
• 作者: Toth, Istvan;Nougayrede, Jean-Philippe;Oswald, Eric
• 通讯作者: Oswald, Eric

Cytolethal distending toxin from Aggregatibacter actinomycetemcomitans induces DNA damage, S/G2 cell cycle arrest and caspase-independent death in budding yeast model.

来自放线菌聚集菌的细胞致死膨胀毒素在芽殖酵母模型中诱导 DNA 损伤、S/G2 细胞周期停滞和不依赖于半胱天冬酶的死亡。

• 发表时间: 2010
• 期刊: Infection and Immunity 78
• 作者: Oranart Matangkasombut;Roongtiwa Wattanawaraporn;Keiko Tsuruda;Masaru Ohara;Motoyuki Sugai;Skorn Mongkolsuk.
• 通讯作者: Skorn Mongkolsuk.