Metabolic strategies of epidermal stem cells

表皮干细胞的代谢策略

• 批准号: 50029202
• 负责人: Professor Dr. Rudolf Wiesner
• 金额: --
• 依托单位: Institut für Vegetative Physiologie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2016-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/50029202?language=en
• 关键词: Metabolic; strategies; epidermal; stem; cells

We showed that epidermal progenitor/stem cells (EPSCs) in vivo are independent of the mitochondrial electron transport chain (ETC) and can use glycolysis alone to cover their energy demand. In the present application, we will use gene expression profiling as well as high-resolution polarography to characterize the metabolic phenotype of highly purified mouse EPSCs and hair follicle bulge stem cells (HFBSCs). We will also determine whether these cells have a capacity for adaption after genetic inactivation of the ETC in vivo. In parallel, we will generate mice with ETC inactivation specifically in HFBSCs and characterize their phenotype to show if our initial findings in EPSCs can be extended to other SCs populations. A mouse model with inactivation of Hypoxia Inducible Factor 1alpha (HIF1alpha) as well as the ETC in EPSCs will show if HIF1alpha is crucial for their metabolic strategy as well as their adaptation.

我们发现，表皮祖细胞/干细胞（EPSC）在体内是独立的线粒体电子传递链（ETC），可以使用糖酵解单独支付其能量需求。在本申请中，我们将使用基因表达谱以及高分辨率极谱法来表征高度纯化的小鼠EPSC和毛囊隆突干细胞（HFBSC）的代谢表型。我们还将确定这些细胞在体内ETC基因失活后是否具有适应能力。同时，我们将产生在HFBSC中特异性ETC失活的小鼠，并表征其表型，以显示我们在EPSC中的初步发现是否可以扩展到其他SC群体。缺氧诱导因子1 α（HIF 1 α）以及EPSC中ETC失活的小鼠模型将显示HIF 1 α是否对其代谢策略以及适应至关重要。