Research of cell detachment behavior from nano-biomaterial surfaces

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Cell sheet engineering using poly(N-isopropylacrylamide)-modified temperature responsive surfaces has been applied to regenerative medicine. Cell attachment/detachment properties strongly depend on the thickness of these polymer layers. However, little is known about the thickness of the temperature responsive polymer layer arising from phase transition under aqueous conditions. In this paper, we characterize different thicknesses of thermoresponsive surfaces using atomic force microscopy, X-ray photoelectron spectroscopy and protein adsorption assay. Furthermore, we discuss the thickness dependency of cell attachment/detachment properties of thermoresponsive polymeric surfaces.