Analysis on regulatory factors and functional mechanisms for the initiator protein of the chromosomal replication

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Initiation of the chromosomal DNA replication is a crucial reaction that regulates the cell proliferation. The active form of Escherichia coli DnaA protein initiates the chromosomal replication, whereas the inactive form does not. This study revealed for the first time that specific DNA sequences (termed DARS) on the chromosome directly activate DnaA. Moreover, it revealed molecular mechanisms in stimulation for the initiation reaction by the DnaA-binding protein DiaA and in post-initiational inactivation of DnaA by Hda protein.