Physiological role of the blood-brain barrier transportsome

建立了质谱法测定转运蛋白含量的方法。测定了构成血脑屏障的脑毛细血管内皮细胞中转运蛋白的表达量和转运蛋白的转运活性。因此，葡萄糖转运蛋白1和多药耐药蛋白1a （mdr1a）的功能在正常情况下能够重建，而mdr1a在炎症条件下的功能则无法重建。也提示转运体参与血脑屏障的部分生理功能。

The quantification method of transporter proteins was established by means of mass spectrometer. The protein expression amounts and transport activities of transporters were determined in brain capillary endothelial cells, which form the blood-brain barrier. As a result, the function of glucose transporter 1 and multidrug resistant protein 1a (mdr1a) was able to be re-constructed in normal conditions, while the function of mdr1a in inflammation conditions was not. It was also suggested that the transportsome was involved in a part of physiological function of the blood-brain barrier.