Visualization and analysis of membrane fusion mechanism during vesicular transport of transportosome by using multi-photon excitation process

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Physiological functions of membrane transporter are supposed to be closely related to vesicular transport. However, membrane fusion mechanism underlying vital functions including exocytosis remains unclear. In order to clarify the molecular basis, we have developed a new light microscopy, two-photon microscopy, which is realized by using non-linear optics, multi-photon excitation process. By using two-photon microscopy, we have established new methods, which enable us to visualize finer structures inside a living organ, and we have obtained new important insights on molecular mechanism of exocytosis, and on physiological and pathological roles of membrane fusion.