Visualization analysis of neurotransmission and exocytosis by using novel fluorescent protein and laser optics technology

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We have developed novel visualization analysis techniques for neurotransmission and exocytosis, and constructed “in vivo” optical imaging methods to advance for elucidation of the molecular basis. We generated fluorescently tagged SNARE proteins, VAMP-Sirius by using a novel fluorescent protein Sirius in order to visualize dynamics of acid vesicles in an osteoclast model. A novel FRET Ca^<2+> sensor, Cameleon-nano protein, visualized Ca^<2+>-dependent exocyosis in pancreatic acinar cells, as well as Ca^<2+> dynamics in neurons in cerebellum. Furthermore, we successfully observed hippocampal neurons in deeper layers in live mouse brains.