Visualization analysis of neurotransmission and exocytosis by using novel fluorescent protein and laser optics technology

我们开发了新的神经传递和胞吐可视化分析技术，并构建了“体内”光学成像方法来推进分子基础的阐明。我们使用一种新的荧光蛋白Sirius生成荧光标记的SNARE蛋白VAMP-Sirius，以便在破骨细胞模型中可视化酸囊泡的动力学。一种新型FRET Ca^<2+>传感器，cameleon -纳米蛋白，可以可视化胰腺腺泡细胞中Ca^<2+>依赖的胞外增生，以及小脑神经元中Ca^<2+>的动态。此外，我们成功地观察了活体小鼠大脑中更深层次的海马神经元。

We have developed novel visualization analysis techniques for neurotransmission and exocytosis, and constructed “in vivo” optical imaging methods to advance for elucidation of the molecular basis. We generated fluorescently tagged SNARE proteins, VAMP-Sirius by using a novel fluorescent protein Sirius in order to visualize dynamics of acid vesicles in an osteoclast model. A novel FRET Ca^<2+> sensor, Cameleon-nano protein, visualized Ca^<2+>-dependent exocyosis in pancreatic acinar cells, as well as Ca^<2+> dynamics in neurons in cerebellum. Furthermore, we successfully observed hippocampal neurons in deeper layers in live mouse brains.