The role of ROP signaling in auxin-dependent oriented cell division

ROP 信号在生长素依赖性细胞分裂中的作用

• 批准号: 501457569
• 负责人: Dr. Prasad Vaddepalli, Ph.D.
• 金额: --
• 依托单位: Zentrum für Molekularbiologie der Pflanzen (ZMBP)
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/501457569?language=en
• 关键词: role; ROP; signaling; auxin; dependent

Cells are fundamental building blocks of plant architecture. Cell shape maintenance and positioning of the wall during cell division are crucial for plant morphogenesis because of cell wall constraints. Orientation of division plane forms the basis for formative events, which determine the cell and tissue diversity during embryonic and post-embryonic development. Thus, understanding the mechanisms employed in this process is of central importance in plant cell and developmental biology. During Arabidopsis embryogenesis the orientation of the cell division plane in symmetric cell divisions is determined by a simple geometric default rule based on minimal surface area. By contrast, asymmetric cell divisions, which result in the formation of new tissue layers, deviate from this simple rule upon transcriptional auxin response. Further analysis on the molecular regulation of this phenomenon revealed MT-associated IQD proteins and signalling components of the small Rho GTPases, namely ROPs. In this project, I will use a combination of approaches involving genetics, molecular biology, cell biology and computational imaging analysis, to address how ROP activity regulates cell shape and division plane orientation during embryo development. I will investigate whether this involves regulation of plasma membrane polarity and cytoskeleton organization, and how these processes correlate with oriented cell division. Furthermore, I will explore how the interaction with ROP downstream signalling components like MT-binding IQDs regulate cell dynamics during early embryo patterning. The outcomes of this project will reveal novel signalling mechanisms at the plasma membrane-cytoskeleton interface that maintain cell shape and orient division planes during plant development.

细胞是植物结构的基本构件。由于细胞壁的限制，细胞分裂过程中细胞形状的维持和细胞壁的定位对植物形态建成至关重要。分裂面的方向是形成事件的基础，决定了胚胎和胚后发育过程中细胞和组织的多样性。因此，了解这一过程中所采用的机制在植物细胞和发育生物学中至关重要。在拟南芥胚胎发生过程中，细胞分裂平面的方向在对称的细胞分裂是由一个简单的几何默认规则的基础上最小的表面积。相比之下，不对称的细胞分裂，导致新的组织层的形成，偏离了这个简单的规则转录生长素的反应。对这种现象的分子调控的进一步分析揭示了MT相关的IQD蛋白和小Rho GTP酶的信号传导组分，即ROP。在这个项目中，我将使用涉及遗传学，分子生物学，细胞生物学和计算成像分析的方法相结合，以解决ROP活动如何调节胚胎发育过程中的细胞形状和分裂平面方向。我将研究这是否涉及质膜极性和细胞骨架组织的调节，以及这些过程如何与定向细胞分裂相关。此外，我将探讨如何与ROP下游信号传导组件的相互作用，如MT结合的IQDs调节细胞动力学在早期胚胎图案。该项目的成果将揭示质膜-细胞骨架界面的新信号机制，这些机制在植物发育过程中维持细胞形状并定向分裂平面。