RAC/ROP signaling in the interaction of Arabidopsis and barley with powdery mildew fungi

拟南芥和大麦与白粉病真菌相互作用中的 RAC/ROP 信号传导

• 批准号: 37334421
• 负责人: Professor Dr. Ralph Hückelhoven
• 金额: --
• 依托单位: Lehrstuhl für Phytopathologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/37334421?language=en
• 关键词: RAC; ROP; signaling; interaction; Arabidopsis

Small monomeric RAC/ROP GTPases are molecular switches in cell polarity and plant defence responses. To identify barley RAC/ROP-interacting proteins (RAC/ROP-IPs), we have carried out a non-targeted yeast two hybrid screening for barley HvRAC1-interacting proteins. This revealed 28 potential interaction partners including promising candidates involved in regulation of RAC/ROP activity, signalling, endocytosis and exocytosis. A HvRAC1-binding kinase and a novel cytoskeleton associated RAC/ROP GTPase activating protein have been found to influence the interaction outcome of barley and Blumeria graminis. These RAC/ROP-IPs are now subject of detailed analysis of their function in interaction of barley and Arabidopsis with powdery mildew fungi. Additionally, we analysed 54 Arabidopsis mutant genotypes corresponding to barley RAC/ROP-IPs for aberrant powdery mildew infection phenotypes. This revealed 7 mutants super-susceptible or partially resistant to fungal pathogenesis. We are going to analyse corresponding barley and Arabidopsis RAC/ROP-IPs in respect to in planta protein-protein interaction and to their role in cellular reprogramming during the plant-fungus interaction.

小型单体RAC/ROP GTPase是细胞极性和植物防御反应中的分子开关。为了识别大麦RAC/ROP相互作用蛋白（RAC/ROP-ips），我们对大麦HVRAC1相互作用蛋白进行了非靶向酵母两种混合筛选。这揭示了28个潜在的相互作用伙伴，包括参与RAC/ROP活性，信号传导，内吞作用和胞吐作用的有前途的候选人。发现HVRAC1结合激酶和新型的细胞骨架相关的RAC/ROP GTPase激活蛋白会影响大麦和bllumeria graminis的相互作用结果。这些RAC/ROP-ips现在对它们在大麦和拟南芥与白粉病真菌相互作用中的功能进行详细分析。此外，我们分析了54种拟南芥突变体基因型，与大麦RAC/ROP-ips相对应，用于异常的白粉病感染表型。这揭示了7种突变体超敏感或部分抗真菌发病机理。我们将分析相应的大麦和拟南芥RAC/ROP-IPS与植物蛋白 - 蛋白质相互作用以及它们在植物 - 膜相互作用过程中的细胞重编程中的作用。