RAC/ROP signaling in the interaction of Arabidopsis and barley with powdery mildew fungi

拟南芥和大麦与白粉病真菌相互作用中的 RAC/ROP 信号传导

• 批准号: 37334421
• 负责人: Professor Dr. Ralph Hückelhoven
• 金额: --
• 依托单位: Lehrstuhl für Phytopathologie
• 依托单位国家: 德国
• 项目类别: Priority Programmes
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2013-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/37334421?language=en
• 关键词: RAC; ROP; signaling; interaction; Arabidopsis

Small monomeric RAC/ROP GTPases are molecular switches in cell polarity and plant defence responses. To identify barley RAC/ROP-interacting proteins (RAC/ROP-IPs), we have carried out a non-targeted yeast two hybrid screening for barley HvRAC1-interacting proteins. This revealed 28 potential interaction partners including promising candidates involved in regulation of RAC/ROP activity, signalling, endocytosis and exocytosis. A HvRAC1-binding kinase and a novel cytoskeleton associated RAC/ROP GTPase activating protein have been found to influence the interaction outcome of barley and Blumeria graminis. These RAC/ROP-IPs are now subject of detailed analysis of their function in interaction of barley and Arabidopsis with powdery mildew fungi. Additionally, we analysed 54 Arabidopsis mutant genotypes corresponding to barley RAC/ROP-IPs for aberrant powdery mildew infection phenotypes. This revealed 7 mutants super-susceptible or partially resistant to fungal pathogenesis. We are going to analyse corresponding barley and Arabidopsis RAC/ROP-IPs in respect to in planta protein-protein interaction and to their role in cellular reprogramming during the plant-fungus interaction.

小单体 RAC/ROP GTP 酶是细胞极性和植物防御反应中的分子开关。为了鉴定大麦 RAC/ROP 相互作用蛋白 (RAC/ROP-IPs)，我们对大麦 HvRAC1 相互作用蛋白进行了非靶向酵母两种杂交筛选。这揭示了 28 个潜在的相互作用伙伴，包括参与 RAC/ROP 活性、信号传导、内吞作用和胞吐作用调节的有前途的候选者。已发现 HvRAC1 结合激酶和新型细胞骨架相关 RAC/ROP GTP 酶激活蛋白可影响大麦和 Blumeria graminis 的相互作用结果。这些 RAC/ROP-IP 现在正在详细分析它们在大麦和拟南芥与白粉病真菌相互作用中的功能。此外，我们还分析了与大麦 RAC/ROP-IP 相对应的 54 种拟南芥突变体基因型的异常白粉病感染表型。这揭示了 7 个突变体对真菌发病机制超级敏感或部分耐药。我们将分析相应的大麦和拟南芥 RAC/ROP-IP 在植物蛋白-蛋白相互作用方面的作用以及它们在植物-真菌相互作用过程中细胞重编程中的作用。