Roles of actin cytoskeletal regulation system in tumor cell migrationand invasion

我们研究了肌动蛋白细胞骨架调控在肿瘤细胞迁移和侵袭中的作用，得到的结果如下：Cofilin及其调控酶Lim-Kinase和Slingshot在Inposodium的形成中起着关键作用。在3D基质中，LIM-K对于间充质细胞向阿米巴细胞的形态转变至关重要。蛋白激酶D1通过Slingshot失活来调节肌动蛋白的重组。我们还开发了LIM-Kinase的抑制剂，并发现这些抑制剂可以抑制肿瘤细胞的侵袭。

We investigated the roles of actin cytoskeletal regulation in tumor cell migration and invasion and obtained the results, as follows : Cofilin and its regulatory enzymes, LIM-kinase and Slingshot, play critical roles in the invadopodium formation. LIM-kinase is critical for the mesenchymal-to-amoeboid cell morphological transition of tunor cells in 3D matrices. Protein kinase D1 regulates actin reorganization through Slingshot inactivation. We also developed inhibitors for LIM-kinase and found that these inhibitors suppressed tumor cell invasion.