High-sensitive biochemical gas sensor based on the gas-biomolecule interaction

基于气体-生物分子相互作用的高灵敏生化气体传感器

• 批准号: 24656252
• 负责人: KUDO Hiroyuki
• 金额: $ 2.08万
• 依托单位: Meiji University (2013)Tokyo Medical and Dental University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656252/
• 关键词: バイオセンサ; ガスセンサ; 環境計測; 生化学

A biosensor using NADH as an electron acceptor was developed. The biosensor measures the presence of the substrate as fluorescence of NADH. In order to enhance the sensitivity to formaldehyde, chemical amplification of NADH was investigated. Aldehyde dehydrogenase produces formic acid and NADH by oxidizing formaldehyde under the presence of NAD+. We co-immobilized formic acid dehydrogenase to re-produce NADH using formic acid. Consequently, the sensor showed sub-ppb level sensitivity to forimaldehyde. Also, real-sample test using photocatalystic TiO2 was carried out. The sensor successfully traced the change of formaldehyde by the effect of photocatalystic reaction in real-time.

开发了使用NADH作为电子受体的生物传感器。生物传感器测量底物作为NADH的荧光的存在。为了增强对甲醛的敏感性，研究了NADH的化学扩增。醛脱氢酶在NAD+存在下通过氧化甲醛氧化而产生甲酸和NADH。我们将甲酸脱氢酶共同磨碎以使用甲酸重新生产NADH。因此，传感器显示出对forimaldehyde的亚ppb水平敏感性。同样，使用光催化tiO2进行了实际样本测试。传感器通过实时的光催化反应的影响成功地追踪了甲醛的变化。

项目成果

NADH-Fluorometric Biochemical Gas Sensor (Bio-Sniffer) for Evaluation of Indoor Air Quality

• DOI: 10.1109/jsen.2013.2257739
• 发表时间: 2013-04
• 期刊: IEEE Sensors Journal
• 影响因子: 4.3
• 作者: H. Kudo;T. Yamashita;K. Miyajima;T. Arakawa;K. Mitsubayashi

NADH-fluorometric biochemical gas sensor (bio-sniffer) for control of indoor air quality

用于控制室内空气质量的 NADH 荧光生化气体传感器（生物嗅探器）

• 发表时间: 2012
• 作者: Kudo H;Yamashita T;Gessei T;Arakawa T;Mitsubayashi K
• 通讯作者: Mitsubayashi K

A highly sensitive formaldehyde gas sensor"bio-sniffer"for monitoring of indoor environment

用于室内环境监测的高灵敏度甲醛气体传感器“生物嗅探器”

• 作者: Ye M;Yamashita T;Gessei T;Miyajima K;Munkhjargal M;Arakawa T;Kudo H;Mitsubayashi K
• 通讯作者: Mitsubayashi K

A direct gaseous ethanol imaging system for analysis of alcohol metabolism from exhaled breath

• DOI: 10.1016/j.snb.2013.05.071
• 发表时间: 2013-09
• 期刊: Sensors and Actuators B-chemical
• 影响因子: 8.4
• 作者: T. Arakawa;Xin Wang;Takumi Kajiro;K. Miyajima;Shuhei Takeuchi;H. Kudo;K. Yano;K. Mitsubayashi

環境中の極微量ホルムアルデヒド計測を目的としたNADH蛍光検出型生化学式ガスセンサ

NADH荧光检测生化气体传感器，用于测量环境中微量甲醛

• 发表时间: 2012
• 作者: 工藤寛之;山下俊文;叶明;宮島久美子;荒川貴博;関口哲志;三林浩二
• 通讯作者: 三林浩二