High-sensitive biochemical gas sensor based on the gas-biomolecule interaction

基于气体-生物分子相互作用的高灵敏生化气体传感器

• 批准号: 24656252
• 负责人: KUDO Hiroyuki
• 金额: $ 2.08万
• 依托单位: Meiji University (2013)Tokyo Medical and Dental University (2012)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Challenging Exploratory Research
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-24656252/
• 关键词: バイオセンサ; ガスセンサ; 環境計測; 生化学

A biosensor using NADH as an electron acceptor was developed. The biosensor measures the presence of the substrate as fluorescence of NADH. In order to enhance the sensitivity to formaldehyde, chemical amplification of NADH was investigated. Aldehyde dehydrogenase produces formic acid and NADH by oxidizing formaldehyde under the presence of NAD+. We co-immobilized formic acid dehydrogenase to re-produce NADH using formic acid. Consequently, the sensor showed sub-ppb level sensitivity to forimaldehyde. Also, real-sample test using photocatalystic TiO2 was carried out. The sensor successfully traced the change of formaldehyde by the effect of photocatalystic reaction in real-time.

研制了一种以NADH为电子受体的生物传感器。生物传感器测量底物的存在作为NADH的荧光。为了提高对甲醛的灵敏度，研究了NADH的化学放大作用。醛脱氢酶通过在NAD+存在下氧化甲醛产生甲酸和NADH。我们共固定化甲酸脱氢酶，利用甲酸再生NADH。因此，该传感器对甲醛显示出亚ppb级的灵敏度。此外，使用光催化TiO2进行了实际样品测试。该传感器成功地利用光催化效应实时跟踪了甲醛的变化。

项目成果

NADH-Fluorometric Biochemical Gas Sensor (Bio-Sniffer) for Evaluation of Indoor Air Quality

• DOI: 10.1109/jsen.2013.2257739
• 发表时间: 2013-04
• 期刊: IEEE Sensors Journal
• 影响因子: 4.3
• 作者: H. Kudo;T. Yamashita;K. Miyajima;T. Arakawa;K. Mitsubayashi

NADH-fluorometric biochemical gas sensor (bio-sniffer) for control of indoor air quality

用于控制室内空气质量的 NADH 荧光生化气体传感器（生物嗅探器）

• 发表时间: 2012
• 作者: Kudo H;Yamashita T;Gessei T;Arakawa T;Mitsubayashi K
• 通讯作者: Mitsubayashi K

A highly sensitive formaldehyde gas sensor"bio-sniffer"for monitoring of indoor environment

用于室内环境监测的高灵敏度甲醛气体传感器“生物嗅探器”

• 作者: Ye M;Yamashita T;Gessei T;Miyajima K;Munkhjargal M;Arakawa T;Kudo H;Mitsubayashi K
• 通讯作者: Mitsubayashi K

環境中の極微量ホルムアルデヒド計測を目的としたNADH蛍光検出型生化学式ガスセンサ

NADH荧光检测生化气体传感器，用于测量环境中微量甲醛

• 发表时间: 2012
• 作者: 工藤寛之;山下俊文;叶明;宮島久美子;荒川貴博;関口哲志;三林浩二
• 通讯作者: 三林浩二

A direct gaseous ethanol imaging system for analysis of alcohol metabolism from exhaled breath

• DOI: 10.1016/j.snb.2013.05.071
• 发表时间: 2013-09
• 期刊: Sensors and Actuators B-chemical
• 影响因子: 8.4
• 作者: T. Arakawa;Xin Wang;Takumi Kajiro;K. Miyajima;Shuhei Takeuchi;H. Kudo;K. Yano;K. Mitsubayashi