Two-photon microscope with integrated photostimulation and electrophysiology modules

我们在这里申请一个双光子显微镜与集成的光刺激和电生理模块的神经科学和心血管研究在维尔茨堡大学的生理学研究所。应用的一个中心领域是使用Ca 2+敏感指标分析神经元网络动力学和突触过程。其他的科学主题，以解决拟议的显微镜包括利钠肽对冠状动脉微循环的影响，以及巨核细胞和血小板的生理和病理生理。拟议的研究项目的指导原则是考虑到生理过程只能在其三维结构的背景下充分理解。为此，该系统配备了一个共振-振镜-振镜扫描仪和一个快速压电致动器，以便即使在多个光学部分也能以高时间和/或空间分辨率测量生物过程。光遗传学方法可以通过专用的光刺激模块集成。该系统还允许进行电生理（膜片钳）和光学记录。所提出的显微镜具有模块化设计，这使得在活体动物在体内和体外制剂的光电生理应用。所要求的设备也将提供给维尔茨堡大学和维尔茨堡大学医院的其他科学家。由于其独特的技术特点，拟议的双光子显微镜将为活细胞成像开辟新的可能性，从而为加强维尔茨堡的生物医学研究做出重要贡献。

We here apply for a two-photon microscope with integrated photostimulation and electrophysiology modules for neuroscientific and cardiovascular research at the Institute of Physiology of the University of Würzburg. A central field of application is the analysis of neuronal network dynamics and synaptic processes using Ca2+-sensitive indicators. Other scientific topics to be addressed by the proposed microscope include the effects of natriuretic peptides on coronary microcirculation as well as the physiology and pathophysiology of megakaryocytes and platelets. A guiding principle of the proposed research projects is the consideration that physiological processes can only be adequately understood in the context of their three-dimensional structure. To this end, the system is equipped with a resonance-galvanometer-galvanometer scanner and a fast piezo actuator in order to enable measurements of biological processes at high temporal and/or spatial resolution even in multiple optical sections. Optogenetic methods can be integrated via a dedicated photostimulation module. The system also allows combined electrophysiological (patch-clamp) and optical recordings to be performed. The proposed microscope has a modular design, which enables optical-electrophysiological applications in both living animals in vivo and in vitro preparations. The requested device will also be made available to other scientists at the University of Würzburg and the University Hospital of Würzburg. Due to its unique technical features, the proposed two-photon microscope will open up new possibilities for live-cell imaging and thus make an important contribution to strengthening biomedical research in Würzburg.