Two-photon microscope with integrated photostimulation and electrophysiology modules

具有集成光刺激和电生理学模块的双光子显微镜

• 批准号: 502670664
• 金额: --
• 依托单位: Julius-Maximilians-Universität Würzburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/502670664?language=en
• 关键词: Two; photon; microscope; integrated; photostimulation

We here apply for a two-photon microscope with integrated photostimulation and electrophysiology modules for neuroscientific and cardiovascular research at the Institute of Physiology of the University of Würzburg. A central field of application is the analysis of neuronal network dynamics and synaptic processes using Ca2+-sensitive indicators. Other scientific topics to be addressed by the proposed microscope include the effects of natriuretic peptides on coronary microcirculation as well as the physiology and pathophysiology of megakaryocytes and platelets. A guiding principle of the proposed research projects is the consideration that physiological processes can only be adequately understood in the context of their three-dimensional structure. To this end, the system is equipped with a resonance-galvanometer-galvanometer scanner and a fast piezo actuator in order to enable measurements of biological processes at high temporal and/or spatial resolution even in multiple optical sections. Optogenetic methods can be integrated via a dedicated photostimulation module. The system also allows combined electrophysiological (patch-clamp) and optical recordings to be performed. The proposed microscope has a modular design, which enables optical-electrophysiological applications in both living animals in vivo and in vitro preparations. The requested device will also be made available to other scientists at the University of Würzburg and the University Hospital of Würzburg. Due to its unique technical features, the proposed two-photon microscope will open up new possibilities for live-cell imaging and thus make an important contribution to strengthening biomedical research in Würzburg.

在这里，我们在温兹堡大学生理学研究所进行神经科学和心血管研究的综合光刺激和电生理模块申请了两光子显微镜。应用的中心字段是使用CA2+敏感指标对神经元网络动力学和突触过程进行分析。拟议的显微镜将要解决的其他科学主题包括纳二硫酸辣椒对冠状动脉微循环以及巨核细胞和血小板的生理学和病理生理学的影响。拟议的研究项目的指导原则是考虑到只有在其三维结构的背景下才能充分理解物理过程。为此，该系统配备了谐振 - 瓦瓦奈仪 - 吉尔瓦奈计扫描仪和快速的压电执行器，以便即使在多个光学部分中也可以在高临时和/或空间分辨率下测量生物学过程。可以通过专用的光刺激模块整合光遗传学方法。该系统还允许进行组合的电生理（贴片钳）和光学记录。所提出的显微镜具有模块化设计，可以在活体内和体外制剂中启用光学循环生理应用。该要求的设备还将提供给Würzburg大学和Würzburg大学医院的其他科学家。由于其独特的技术特征，提出的两光子显微镜将为实时成像开辟新的可能性，从而为加强Würzburg的生物医学研究做出重要贡献。