ESI-IM-mass spectrometer

ESI-IM质谱仪

• 批准号: 503478512
• 金额: --
• 依托单位: Universität Osnabrück
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/503478512?language=en
• 关键词: ESI; IM; mass; spectrometer

The scientific questions of the groups involved in this application focus on the analysis of specific lipid and protein composition and dynamics at the molecular, subcellular and cellular level. Thus, analyses of individual proteins, flux measurements of specific metabolites or even individual lipid species in their natural environment (in the cell, organelle or membrane) are necessary. Only by detecting the modification of these molecules by the cell (post-translational modifications such as phosphorylation of proteins, structural modifications on lipids such as degree of saturation, cyclizations or conversion, the mechanisms of action can be determined.This requires mass spectrometers that identify and characterize these molecules with increasing sensitivity, accuracy and targeting in a considerable biological background. In recent years in particular, considerable further developments have been achieved in the mass accuracy, speed and sensitivity of the corresponding mass spectrometers. In addition, "ion mobility" (IM) has been established in almost all instruments on the market. With the help of this technology, molecules can be separated again in the MS itself before their actual mass determination. This allows the separation or characterization not only of isobaric or very similar molecules, but also a much better characterization of complex sample mixtures.Last but not least, significant instrumental improvements in the upstream separation systems (LC) and new algorithms in the downstream evaluation systems (software) have also been introduced.Since it is not possible to upgrade existing systems to the latest technology, a new LC-IM mass spectrometer is requested here in order to be able to address the current scientific questions of the deparmtent of biology/chemistry in sufficient depth.

参与该应用的小组的科学问题集中在分析特定的脂质和蛋白质组成以及分子、亚细胞和细胞水平的动力学。因此，有必要分析单个蛋白质，测量特定代谢物或甚至单个脂质物质在其自然环境中（细胞，细胞器或膜中）的通量。只有通过检测细胞对这些分子的修饰（翻译后修饰，如蛋白质的磷酸化，脂质的结构修饰，如饱和度，环化或转化），才能确定作用机制。这需要质谱仪在相当大的生物背景下以更高的灵敏度，准确度和靶向来鉴定和表征这些分子。特别是近年来，相应的质谱仪在质量精度、速度和灵敏度方面取得了相当大的进一步发展。此外，市场上几乎所有仪器都已建立了“离子迁移率”（IM）。在这项技术的帮助下，分子可以在MS本身中再次分离，然后再进行实际质量测定。这不仅可以分离或表征同量异位素或非常相似的分子，而且可以更好地表征复杂的样品混合物。最后但并非最不重要的是，上游分离系统（LC）的显著仪器改进和下游评估系统的新算法（软件）也已经引入。由于不可能将现有系统升级到最新技术，本文要求一种新的LC-IM质谱仪，以便能够足够深入地解决生物/化学部门当前的科学问题。