ESI-IM-mass spectrometer

参与这一应用的小组的科学问题侧重于在分子、亚细胞和细胞水平上分析特定的脂和蛋白质组成和动力学。因此，在自然环境(细胞、细胞器或细胞膜)中对单个蛋白质进行分析、对特定代谢物甚至单个脂类进行通量测量是必要的。只有通过检测细胞对这些分子的修饰(翻译后修饰，如蛋白质的磷酸化，脂类的结构修饰，如饱和度，环化或转化)，才能确定作用机制。这需要质谱仪在相当大的生物背景下以越来越高的灵敏度、准确性和靶向性来识别和表征这些分子。特别是近年来，相应的质谱计在质量精度、速度和灵敏度方面取得了长足的发展。此外，市场上几乎所有的仪器都建立了“离子迁移率”(IM)。在这项技术的帮助下，分子可以在实际质量测定之前在MS本身中再次分离。这不仅可以分离或表征等压或非常相似的分子，而且还可以更好地表征复杂的样品混合物。最后但同样重要的是，上游分离系统(LC)的重大仪器改进和下游评估系统(软件)中的新算法也得到了介绍。由于无法将现有系统升级到最新技术，因此需要一台新的LC-IM质谱仪，以便能够足够深入地解决当前生物/化学分离的科学问题。

The scientific questions of the groups involved in this application focus on the analysis of specific lipid and protein composition and dynamics at the molecular, subcellular and cellular level. Thus, analyses of individual proteins, flux measurements of specific metabolites or even individual lipid species in their natural environment (in the cell, organelle or membrane) are necessary. Only by detecting the modification of these molecules by the cell (post-translational modifications such as phosphorylation of proteins, structural modifications on lipids such as degree of saturation, cyclizations or conversion, the mechanisms of action can be determined.This requires mass spectrometers that identify and characterize these molecules with increasing sensitivity, accuracy and targeting in a considerable biological background. In recent years in particular, considerable further developments have been achieved in the mass accuracy, speed and sensitivity of the corresponding mass spectrometers. In addition, "ion mobility" (IM) has been established in almost all instruments on the market. With the help of this technology, molecules can be separated again in the MS itself before their actual mass determination. This allows the separation or characterization not only of isobaric or very similar molecules, but also a much better characterization of complex sample mixtures.Last but not least, significant instrumental improvements in the upstream separation systems (LC) and new algorithms in the downstream evaluation systems (software) have also been introduced.Since it is not possible to upgrade existing systems to the latest technology, a new LC-IM mass spectrometer is requested here in order to be able to address the current scientific questions of the deparmtent of biology/chemistry in sufficient depth.