X-ray micro computed-tomography scanner

X 射线微型计算机断层扫描仪

• 批准号: 503673978
• 金额: --
• 依托单位: Ruprecht-Karls-Universität Heidelberg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/503673978?language=en
• 关键词: ray; micro; computed; tomography; scanner

Biological laboratories are routinely equipped with imaging tools such as confocal, inverse, light-sheet, and super-resolution microscopes. While all these microscopes are vital for addressing biological questions, they do not fulfill the need for high throughput whole-body imaging of small organisms, organs, patient’s tissue and biopsy samples. Since its development in 1972, x-ray computed tomography became the standard medical imaging device due to its robustness and low costs. The performance of table-top laboratory x-ray CT scanners has since then significantly improved in spatial resolution, providing 500 nm in resolution and sample throughput (tens of samples per day). Over the last years, x-ray computed tomography became the method of choice for 3-D structural imaging of organisms and tissues and at single cell resolution. Due to high penetration, the samples can be observed non-destructively - within an (artificial) physical environment, like plant roots in soil or tissues within biomaterials. Correlative approaches link specific proteins of interest observed via fluorescence microscopy with structural information provided by x-rays. Furthermore, recent approaches for hierarchical imaging show that x-ray tomography is extremely useful to locate a specific region of interest for further investigation by electron microscopy and super-resolution microscopy, increasing fidelity of the obtained results and reducing the need for sample sectioning and associated time and costs. The requested x-ray microCT scanner is a state-of-the-art instrument with the high spatial resolution (of 500 nm), throughput (automatic sampling of 16 probes), large field of view to accommodate large specimens and reconstruction algorithms among existing x-ray microCT scanners. As described in section 3, it will address the need of comparative structural analysis on one hand, and it will improve the workflow upstream of other microscope techniques such as light and electron microscopy on the other hand.

生物实验室通常配备有成像工具，如共聚焦，倒置，光片和超分辨率显微镜。虽然所有这些显微镜对于解决生物学问题至关重要，但它们不能满足对小生物体、器官、患者组织和活检样本进行高通量全身成像的需求。自1972年开发以来，X射线计算机断层扫描由于其稳健性和低成本而成为标准的医学成像设备。从那时起，台式实验室X射线CT扫描仪的性能在空间分辨率方面得到了显着改善，分辨率和样品吞吐量（每天数十个样品）达到500 nm。在过去的几年中，X射线计算机断层扫描成为生物体和组织的三维结构成像和单细胞分辨率的首选方法。由于高穿透性，可以在（人工）物理环境中非破坏性地观察样品，如土壤中的植物根或生物材料中的组织。相关方法将通过荧光显微镜观察到的特定蛋白质与X射线提供的结构信息联系起来。此外，最近的分层成像的方法表明，X射线断层扫描是非常有用的，以定位一个特定的区域的兴趣，进一步调查的电子显微镜和超分辨率显微镜，提高保真度所获得的结果，并减少需要的样品切片和相关的时间和成本。所要求的X射线microCT扫描仪是一种最先进的仪器，具有高空间分辨率（500 nm）、吞吐量（16个探头的自动采样）、大视场以适应大型标本和现有X射线microCT扫描仪中的重建算法。如第3节所述，它一方面将满足比较结构分析的需要，另一方面将改善其他显微镜技术（如光学和电子显微镜）的上游工作流程。