ShEEP Request For A Pre-Clinical In-Vivo X-Ray Micro Computed-Tomography Scanner

ShEEP 请求临床前体内 X 射线微型计算机断层扫描仪

• 批准号: 10178581
• 负责人: GEORGE R. BECK
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10178581
• 关键词: 3-Dimensional; Anesthesia procedures; Animals; Award; Biocompatible Materials; Biological; Bone Density; Bone Diseases; Bone Regeneration; Bone Tissue; Bone structure; Collaborations; Collection; Computed Tomography Scanners; Computer Systems; Computer software; Contrast Media; Cost Savings; Custom; Data; Data Storage and Retrieval; Databases; Degenerative polyarthritis; Development; Disease; Doctor of Philosophy; Dose; Dual-Energy X-Ray Absorptiometry; Equipment; Evaluation; Floor; Fracture; Frequencies; Funding; Generations; Hand; Health; Heart; Heating; Homeostasis; Hormones; Image; In Vitro; Joints; Laboratory Animals; Longitudinal Studies; Low Dose Radiation; Measurement; Measures; Medical; Memory; Methodology; Methods; Monitor; Mus; Musculoskeletal; Names; Noise; Operative Surgical Procedures; Oryctolagus cuniculus; Osteoporotic; Pennsylvania; Productivity; Protocols documentation; Quality Control; Regenerative research; Research; Research Personnel; Resolution; Rodent; Rodent Model; Roentgen Rays; Sample Size; Sampling; Savings; Scanning; Schedule; Secure; Sheep; Skeleton; Software Tools; Specimen; Speed; Structure; Switzerland; System; Techniques; Technology; Temperature; Therapeutic Agents; Three-dimensional analysis; Time; Tissues; Touch sensation; Training; Transgenic Animals; Transgenic Mice; Tube; Vertebral column; Veterans; Visualization; Work; animal colony; animal facility; archive data; bone; bone quality; cortical bone; cytokine; data archive; data management; density; detector; drug development; experience; high resolution imaging; improved; in vivo; indexing; instrument; microCT; mouse model; operation; pre-clinical; programs; reconstruction; recruit; repaired; respiratory; response; substantia spongiosa; tissue regeneration; tissue repair; tool

Abstract We are requesting funds for the purchase of a state-of-the-art in vivo micro Computed Tomography (microCT) scanner for non-invasive, nondestructive, volumetric imaging and analysis of the skeleton from live animals ranging in size from mice to rabbits. The vivaCT80 is manufactured by Scanco Medical AG (Brutisellen, Switzerland) and distributed by Scanco USA, Inc. (King of Prussia, Pennsylvania, USA). The system is capable of acquiring and analyzing three-dimensional images of the skeleton and vasculature of live animals with a voxel size as small as 5µm while maintaining a relatively low radiation dose. The combination of low dose radiation, high speed, and a wide-ranging resolution and field of view in the vivaCT80 are made possible by an 8192 pixel wide detector. The vivaCT80 accommodates a wider range of animal/specimen sizes (90mm bore size with an 80mm field of view) than most competing high-resolution instruments. The x-ray tube is capable of peak energies from 30 kVp to 70 kVp which improves image quality (contrast-to-noise ratio, CNR) and reduces scan time and dose for both high- and low-density specimens The scanner includes an integrated touch display, heating system, animal temperature monitoring, observation camera, respiratory gating,heart beat frequency monitoring, and ports for anesthesia and contrast media, and all software needed for standard and customized volumetric and structural analyses. This instrument will be utilized by five major users, all VA funded with laboratories and animal colonies at the Atlanta VAMC; Drs. George R Beck Jr. (PI) (PhD), Neale Weitzmann (PhD), Hicham Drissi (PhD), Nick Willet (PhD), and Steven Presciutti (MD). In addition to 4 VA merits and 1 VA CDA award, these investigators also are funded by 3 R01s, 3 R21s, DOD, Score, and U54 projects, among others, all focused on musculoskeletal research. The research programs range in focus from diseases of bone homeostasis (i.e. osteoporotic disease), fracture repair, osteoarthritis, biomaterials for bone regeneration and tissue repair, and drug development for improved spine fusion. The equipment will be located in the animal facility on the 4th floor of Building A at the Atlanta VAMC (Decatur GA) where all five major users have animal colonies. The in vivo microCT will signficantly facilitate each of these projects focused on pre-clinical rodent models of bone disease and repair and be a great assest to the rapidly expanding Musculoskeletal group at the Atlanta VAMC. The advantages of having an in vivo microCT are several. 1) All five major users utilize transgenic mouse models It would make possible to expand the ongoing and planned studies with in vivo data about bone structure, a capability which is currently not on hand. 2) The greater sensitivity and precision of in vivo microCT over other methods would results in increased group separation and thus increased ability to interpret the data in a conclusive manner. 3) In many cases it will be possible to decrease the sample size and duration of the studies with considerable financial savings. 4) We would be able to conduct our studies at a much faster pace increasing productivity. 5) The increased capability of in vivo microCT would provide additional opportunity for collaborations and recruiting, training of new investigators and opening new fields of research.

摘要 我们正在申请资金购买最先进的活体微型计算机断层扫描 (MicroCT)扫描仪，用于对活体骨骼进行无创、无损、体积成像和分析 动物大小不一，从老鼠到兔子。VivaCT80由Sanco Medical AG制造 (瑞士Brutisellen)，由Sanco USA，Inc.(美国宾夕法尼亚州普鲁士国王)发行。这个 该系统能够获取和分析活体骨骼和血管的三维图像 体素大小小至5微米的动物，同时保持相对较低的辐射剂量。这两种技术的结合 在vivaCT80中进行了低剂量辐射、高速度和宽范围的分辨率和视场 可由8192像素宽的探测器实现。VivaCT80可适应更大范围的动物/标本大小 (孔径90 mm，视场80 mm)比大多数竞争对手的高分辨率仪器都要高。X-射线管 峰值能量从30kVp到70kVp，可提高图像质量(对比度噪声比，CNR) 并减少了高密度和低密度样本的扫描时间和剂量 触摸屏、加热系统、动物体温监测、观察摄像头、呼吸门控、心脏 节拍频率监测，麻醉和造影剂端口，以及标准所需的所有软件 以及定制的体积和结构分析。 该仪器将由五个主要用户使用，所有退伍军人管理局都由实验室和动物群体资助，地址为 亚特兰大VAMC；小乔治·R·贝克博士(PI)(博士)，Neale Weitzmann(博士)，Hicham Drissi(博士)，Nick Willet(博士)和Steven Presciutti(医学博士)。除了4项退伍军人管理局荣誉和1项退伍军人事务部CDA奖外，这些调查人员 还由3个R01、3个R21、国防部、SCORE和U54等项目资助，所有这些项目都集中在 肌肉骨骼研究。研究项目的重点范围从骨骼动态平衡疾病(即 骨质疏松症)，骨折修复，骨关节炎，骨再生和组织修复的生物材料，以及 用于改善脊柱融合的药物开发。这些设备将放置在4楼的动物设施中 位于亚特兰大VAMC(佐治亚州迪凯特)的A号楼，所有五个主要用户都有动物栖息地。 体内显微CT将极大地促进这些专注于临床前啮齿动物模型的项目 关于骨骼疾病和修复的研究，是对亚特兰大迅速扩大的肌肉骨骼组的极大考验 VAMC。拥有活体显微CT的好处有几个。1)所有五个主要用户都使用转基因 小鼠模型，这将使正在进行的和计划中的关于骨骼的研究成为可能 结构，这是目前尚不具备的功能。2)体内MicroCT的灵敏度和精确度更高 与其他方法相比，将导致更多的群体分离，从而提高解释数据的能力 以决定性的方式。3)在许多情况下，可以减少样本大小和持续时间 有相当大的经济节省的研究。我们将能够以更快的速度进行我们的研究 提高工作效率。5)体内MicroCT能力的增强将为以下方面提供额外的机会 合作和招聘，培训新的研究人员，开辟新的研究领域。