ShEEP Request For A Pre-Clinical In-Vivo X-Ray Micro Computed-Tomography Scanner

ShEEP 请求临床前体内 X 射线微型计算机断层扫描仪

• 批准号: 10178581
• 负责人: GEORGE R. BECK
• 金额: --
• 依托单位: VETERANS HEALTH ADMINISTRATION
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-10-01 至 2021-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10178581
• 关键词: 3-Dimensional; Anesthesia procedures; Animals; Award; Biocompatible Materials; Biological; Bone Density; Bone Diseases; Bone Regeneration; Bone Tissue; Bone structure; Collaborations; Collection; Computed Tomography Scanners; Computer Systems; Computer software; Contrast Media; Cost Savings; Custom; Data; Data Storage and Retrieval; Databases; Degenerative polyarthritis; Development; Disease; Doctor of Philosophy; Dose; Dual-Energy X-Ray Absorptiometry; Equipment; Evaluation; Floor; Fracture; Frequencies; Funding; Generations; Hand; Health; Heart; Heating; Homeostasis; Hormones; Image; In Vitro; Joints; Laboratory Animals; Longitudinal Studies; Low Dose Radiation; Measurement; Measures; Medical; Memory; Methodology; Methods; Monitor; Mus; Musculoskeletal; Names; Noise; Operative Surgical Procedures; Oryctolagus cuniculus; Osteoporotic; Pennsylvania; Productivity; Protocols documentation; Quality Control; Regenerative research; Research; Research Personnel; Resolution; Rodent; Rodent Model; Roentgen Rays; Sample Size; Sampling; Savings; Scanning; Schedule; Secure; Sheep; Skeleton; Software Tools; Specimen; Speed; Structure; Switzerland; System; Techniques; Technology; Temperature; Therapeutic Agents; Three-dimensional analysis; Time; Tissues; Touch sensation; Training; Transgenic Animals; Transgenic Mice; Tube; Vertebral column; Veterans; Visualization; Work; animal colony; animal facility; archive data; bone; bone quality; cortical bone; cytokine; data archive; data management; density; detector; drug development; experience; high resolution imaging; improved; in vivo; indexing; instrument; microCT; mouse model; operation; pre-clinical; programs; reconstruction; recruit; repaired; respiratory; response; substantia spongiosa; tissue regeneration; tissue repair; tool

Abstract We are requesting funds for the purchase of a state-of-the-art in vivo micro Computed Tomography (microCT) scanner for non-invasive, nondestructive, volumetric imaging and analysis of the skeleton from live animals ranging in size from mice to rabbits. The vivaCT80 is manufactured by Scanco Medical AG (Brutisellen, Switzerland) and distributed by Scanco USA, Inc. (King of Prussia, Pennsylvania, USA). The system is capable of acquiring and analyzing three-dimensional images of the skeleton and vasculature of live animals with a voxel size as small as 5µm while maintaining a relatively low radiation dose. The combination of low dose radiation, high speed, and a wide-ranging resolution and field of view in the vivaCT80 are made possible by an 8192 pixel wide detector. The vivaCT80 accommodates a wider range of animal/specimen sizes (90mm bore size with an 80mm field of view) than most competing high-resolution instruments. The x-ray tube is capable of peak energies from 30 kVp to 70 kVp which improves image quality (contrast-to-noise ratio, CNR) and reduces scan time and dose for both high- and low-density specimens The scanner includes an integrated touch display, heating system, animal temperature monitoring, observation camera, respiratory gating,heart beat frequency monitoring, and ports for anesthesia and contrast media, and all software needed for standard and customized volumetric and structural analyses. This instrument will be utilized by five major users, all VA funded with laboratories and animal colonies at the Atlanta VAMC; Drs. George R Beck Jr. (PI) (PhD), Neale Weitzmann (PhD), Hicham Drissi (PhD), Nick Willet (PhD), and Steven Presciutti (MD). In addition to 4 VA merits and 1 VA CDA award, these investigators also are funded by 3 R01s, 3 R21s, DOD, Score, and U54 projects, among others, all focused on musculoskeletal research. The research programs range in focus from diseases of bone homeostasis (i.e. osteoporotic disease), fracture repair, osteoarthritis, biomaterials for bone regeneration and tissue repair, and drug development for improved spine fusion. The equipment will be located in the animal facility on the 4th floor of Building A at the Atlanta VAMC (Decatur GA) where all five major users have animal colonies. The in vivo microCT will signficantly facilitate each of these projects focused on pre-clinical rodent models of bone disease and repair and be a great assest to the rapidly expanding Musculoskeletal group at the Atlanta VAMC. The advantages of having an in vivo microCT are several. 1) All five major users utilize transgenic mouse models It would make possible to expand the ongoing and planned studies with in vivo data about bone structure, a capability which is currently not on hand. 2) The greater sensitivity and precision of in vivo microCT over other methods would results in increased group separation and thus increased ability to interpret the data in a conclusive manner. 3) In many cases it will be possible to decrease the sample size and duration of the studies with considerable financial savings. 4) We would be able to conduct our studies at a much faster pace increasing productivity. 5) The increased capability of in vivo microCT would provide additional opportunity for collaborations and recruiting, training of new investigators and opening new fields of research.

摘要 我们正在申请资金购买最先进的活体微型计算机断层扫描仪 （microCT）扫描仪，用于对活体骨骼进行非侵入性、非破坏性、体积成像和分析 从老鼠到兔子大小不等的动物。vivaCT 80由Szero Medical AG生产 （Brutisellen，Switzerland），并由SwitzeroUSA，Inc. (King of Prussia，Pennsylvania，USA）。的 系统能够采集和分析活体骨骼和脉管系统的三维图像 体素尺寸小至5µm的动物，同时保持相对较低的辐射剂量。的组合 vivaCT 80具有低剂量辐射、高速度、宽范围分辨率和视场 可以通过8192像素宽的检测器。vivaCT 80适用于更广泛的动物/标本尺寸 (90mm孔径尺寸，80 mm视场）。x射线管 能够达到30 kVp至70 kVp的峰值能量，从而提高图像质量（对比度噪声比，CNR） 并减少扫描时间和高密度和低密度样本的剂量扫描仪包括集成的 触摸显示器、加热系统、动物体温监测、观察摄像头、呼吸门控、心脏 拍频监测，麻醉和造影剂端口，以及标准所需的所有软件 以及定制的体积和结构分析。 该仪器将由五个主要用户使用，所有VA都由实验室和动物群资助， 亚特兰大VAMC;小乔治R贝克博士(PI)（博士），Neale Weitzmann（博士），Hicham Drissi（博士），Nick Willet（PhD）和Steven Presciutti（MD）。除了4个VA优点和1个VA CDA奖，这些研究人员 此外，还得到了3个R 01、3个R21、国防部、Score和U 54等项目的资助，这些项目的重点都是 肌肉骨骼研究研究项目的范围从骨稳态疾病（即， 骨关节炎疾病）、骨折修复、骨关节炎、用于骨再生和组织修复的生物材料，以及 用于改善脊柱融合的药物开发。设备将位于4楼的动物设施中 亚特兰大VAMC（佐治亚州迪凯特）的A楼，所有五个主要用户都有动物群。 体内microCT将显著促进这些项目中的每一个，重点是临床前啮齿动物模型 骨疾病和修复，并成为一个伟大的assest迅速扩大肌肉骨骼集团在亚特兰大 VAMC。具有体内microCT的优点有几个。1)所有五个主要用户都利用转基因技术 小鼠模型这将有可能扩大正在进行的和计划的研究与体内数据有关的骨 这是一种目前还没有的能力。2)体内microCT的灵敏度和精度更高 与其他方法相比，将导致组分离增加，从而提高解释数据的能力 以决定性的方式。3)在许多情况下，可以减少样本量和持续时间 研究，节省了大量的资金。4)我们将能够以更快的速度进行研究 提高生产力。5)体内microCT能力的提高将为以下方面提供额外的机会： 合作和招聘，培训新的研究人员和开辟新的研究领域。