Design and fabrication of structured microfibers for the development of biomimetic tissues

用于开发仿生组织的结构化微纤维的设计和制造

• 批准号: 504195013
• 负责人: Professor Dr. Ghulam Destgeer
• 金额: --
• 依托单位: Professur Control and Manipulation of Microscale Living Objects
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 2022-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/504195013?language=en
• 关键词: Design; fabrication; structured; microfibers; development

Shaping multiple materials in complex cross-sectional structures will provide numerous advantages in tissue engineering, such as precise control over the position, and interaction of different biomaterials, which will result in increasing the proliferation, and differentiation of cells. Moreover, multi-material arrangement in structured cross-sections will result in mimicking the hierarchical architecture of biological tissues in the organ-on-chip models for drug screening. However, the current fabrication technology face challenges in incorporating multiple materials in complex shapes. In this project, we will propose a microfiber technology for the fabrication of structured microfibers, with multiple materials organized in complex cross-sectional structures. By coflowing four precursor solutions through 3D printed microfluidic devices and curing them with UV exposure, the structured microfibers will be fabricated. The aim of this project will be to engineer the cross-sectional structures of microfibers by tailoring the design of microfluidic devices and the flow rate of precursor solutions in such a way that different combinations and integration areas of material interactions can be established. Firstly, microfluidic devices will be designed to shape multiple materials in eight cross-sectional structures, including concentric, stacked, I-shaped, T-shaped, sandwich, circular-core, multiple-cores, and plus-core cross-sections. Secondly, the polymer precursor solutions will be prepared by varying compositions of polymer solutions, solvent and photo-initiator and the material properties will be determined. Thirdly, the microfluidic devices will be 3D printed and the experimental setup will be established to fabricate microfibers with different cross-sections. The experimental conditions will be optimized to fabricate microfiber in uniform and non-uniform cross-sectional structures. The end goal of this project will be to realize a strategy for the controlled fabrication of a specific cross-sectional structure of microfiber through the 3D printed microfluidic device. After the completion of this project, we will be expanding it for the fabrication of in-vitro tissue models which will serve as organ-on-chip models for drug screening.

将多种材料成形为复杂的横截面结构将在组织工程中提供许多优点，例如对位置的精确控制，以及不同生物材料的相互作用，这将导致增加细胞的增殖和分化。此外，在结构化截面中的多材料布置将导致在用于药物筛选的器官芯片模型中模仿生物组织的分级结构。然而，当前的制造技术在将多种材料结合成复杂形状方面面临挑战。在这个项目中，我们将提出一种用于制造结构化微纤维的微纤维技术，其中多种材料以复杂的横截面结构组织。通过使四种前体溶液共流通过3D打印的微流体设备并通过UV曝光固化它们，将制造结构化的微纤维。该项目的目的是通过定制微流体设备的设计和前体溶液的流速来设计微纤维的横截面结构，以便建立材料相互作用的不同组合和集成区域。首先，微流控器件将被设计成以八种横截面结构成形多种材料，包括同心、堆叠、I形、T形、三明治、圆形芯、多芯和加芯横截面。其次，通过改变聚合物溶液、溶剂和光引发剂的组成来制备聚合物前体溶液，并确定材料性质。第三，将3D打印微流体装置，并建立实验装置，以制造具有不同横截面的微纤维。优化实验条件，制备均匀和非均匀截面结构的超细纤维。该项目的最终目标是通过3D打印微流体设备实现微纤维特定横截面结构的受控制造策略。在这个项目完成后，我们将扩大它的体外组织模型的制造，将作为药物筛选的器官芯片模型。