Application of new analytical techniques for the comprehensive chemical characterization of selected herbal remedies and evaluation of their hepatoprotective effect

越来越多的抗生素耐药细菌正在出现，现有的药物在许多其他疾病中也显示出强烈的副作用或患者依赖的效果。也许古老的僧侣医学知识可以帮助取得进一步的进步。用欧洲药用植物治疗肝病在僧侣医学中有悠久的传统。然而，需要科学数据来支持这些治疗的积极效果。目前，关于用于寺院药物的植物及其化学成分的信息很少。然而，药用植物的生物活性与其化学成分直接相关，因此深入的化学表征对于了解哪些化合物具有有益的作用是必要的。然而，到目前为止，由于这些样品极其复杂，用目前的分析技术很难捕捉到它们的全部组成，因此缺乏信息。在本项目中，提出了一种创新的2D-LC装置，该装置具有新型的双离子源，用于分析中草药中不同类别的化合物的最大值。因此，一种具有两个可切换的不同第二维度的综合二维高效液相色谱(LCxLC)将被用来实现与经典的LCxLC相比进一步的分离性能改进。此外，可切换的双离子源将显著提高电离效率。通过这种方式有望获得高度的鉴定，将对草药提取物的体外保肝活性进行评估，并确定具有生物活性的化合物，为千年传统提供科学知识。

More and more antibiotic-resistant bacteria are appearing, and the existing drugs also show strong side effects or patient-dependent effects in many other diseases. Perhaps the ancient knowledge of monastic medicine can help to make further progress. The treatment of liver diseases with European medicinal plants has a centuries-old tradition in monastic medicine. However, there is a need for scientific data to support the positive effects of these treatments. Currently, little information is available on the plants used in monastic medicine and their chemical compositions. However, the bioactive effects of medicinal plants are directly related to their chemical composition, so in-depth chemical characterisation is imperative to understand which compounds are responsible for the beneficial effects. So far, however, there has been a lack of information because these samples are extremely complex and it is very difficult to capture their full composition with current analytical techniques. In this project, an innovative 2D-LC setup with a novel dual ion source is proposed to analyse the maximum of the different compound classes in the herbal drugs. Thereby, a comprehensive two-dimensional HPLC (LCxLC) with two switchable different second dimensions will be used to realise a further separation performance improvement compared to classical LCxLC. In addition, a switchable dual ion source will significantly increase the efficiency of ionisation. With the high degree of identification expected in this way, the in vitro hepatoprotective activity of the herbal extracts will be evaluated and the compounds responsible for the bioactive effect will be determined to provide scientific knowledge to a thousand-year-old tradition.