Application of new analytical techniques for the comprehensive chemical characterization of selected herbal remedies and evaluation of their hepatoprotective effect

应用新分析技术对选定草药进行综合化学表征并评估其保肝作用

• 批准号: 504370143
• 负责人: Professor Dr. Oliver Schmitz
• 金额: --
• 依托单位: AG Applied Analytical Chemistry
• 依托单位国家: 德国
• 项目类别: Research Grants
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/504370143?language=en
• 关键词: Application; new; analytical; techniques; comprehensive

More and more antibiotic-resistant bacteria are appearing, and the existing drugs also show strong side effects or patient-dependent effects in many other diseases. Perhaps the ancient knowledge of monastic medicine can help to make further progress. The treatment of liver diseases with European medicinal plants has a centuries-old tradition in monastic medicine. However, there is a need for scientific data to support the positive effects of these treatments. Currently, little information is available on the plants used in monastic medicine and their chemical compositions. However, the bioactive effects of medicinal plants are directly related to their chemical composition, so in-depth chemical characterisation is imperative to understand which compounds are responsible for the beneficial effects. So far, however, there has been a lack of information because these samples are extremely complex and it is very difficult to capture their full composition with current analytical techniques. In this project, an innovative 2D-LC setup with a novel dual ion source is proposed to analyse the maximum of the different compound classes in the herbal drugs. Thereby, a comprehensive two-dimensional HPLC (LCxLC) with two switchable different second dimensions will be used to realise a further separation performance improvement compared to classical LCxLC. In addition, a switchable dual ion source will significantly increase the efficiency of ionisation. With the high degree of identification expected in this way, the in vitro hepatoprotective activity of the herbal extracts will be evaluated and the compounds responsible for the bioactive effect will be determined to provide scientific knowledge to a thousand-year-old tradition.

越来越多的耐药菌出现，现有药物在许多其他疾病中也表现出强烈的副作用或患者依赖效应。也许寺院医学的古老知识可以帮助取得进一步的进展。用欧洲药用植物治疗肝病在修道院医学中有着数百年的传统。然而，需要科学数据来支持这些治疗的积极效果。目前，关于寺院药物中使用的植物及其化学成分的信息很少。然而，药用植物的生物活性与它们的化学成分直接相关，因此深入的化学特征是必要的，以了解哪些化合物负责有益的效果。然而，到目前为止，还缺乏信息，因为这些样品非常复杂，用现有的分析技术很难捕捉到它们的全部成分。在本项目中，提出了一种具有新型双离子源的创新2D-LC设置，以分析草药中不同化合物类别的最大值。因此，一种具有两个可切换的不同二维的综合二维高效液相色谱（LCxLC）将被用于实现与传统的LCxLC相比进一步提高分离性能。此外，一个可切换的双离子源将显著提高电离效率。随着这种方法的高度鉴定，将评估草药提取物的体外肝保护活性，并确定产生生物活性作用的化合物，为千年传统提供科学知识。