TIRF Mikroscop with laser illuminaion and two CMOS cameras

带激光照明和两个 CMOS 相机的 TIRF 显微镜

• 批准号: 504444381
• 金额: --
• 依托单位: Albert-Ludwigs-Universität Freiburg
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/504444381?language=en
• 关键词: TIRF; Mikroscop; laser; illuminaion; two

Our studies seek to understand how the spatial organization and assembly dynamics of plasma membrane signaling pathways control T cell activation and functions. The research is based on our findings that plasma membrane proteins are localized in nanometer-sized domains. Our studies revealed that T cell activation is dynamically controlled by the spatial relationships of distinct nanodomains, the assembly of signaling complexes within them and translocation of signaling molecules between them. We study the underlying molecular mechanisms using multidisciplinary strategies that combine single molecule microscopy (i.e. single particle tracking and super-resolution) with biochemical, biophysical, and cell biological approaches. Specifically, we are interested in T cell receptor (TCR) and the inhibitory program cell death protein (PD-1) pathways during T cell activation and anti-tumor responses. Our ultimate goal is to identify novel control principles in T cell signaling pathways and use them to modify T cell responses for future immunotherapies. We request an advanced Total Internal Reflection (TIRF) microscope, which is essential to our newly established laboratory at the Albert-Ludwigs-Universität Freiburg and for realizing our current and future research goals. The requested microscope has the following capabilities: (i) Total Internal Reflection Microscopy, where illumination is limited to the contact site between cells and glass surfaces. (ii) Single molecule detection for super resolution imaging and particle tracking to analyze protein distributions and dynamics. Images are acquired with two CMOS cameras with high dynamic range and large fields of view. (iii) Localized laser control for photo-manipulation to measure protein dynamics and dispersion. Up to six medium powered lasers covering a broad range of illumination wavelengths. (iv) Widefield illumination for fast acquisition and calcium analyses. (v) Environmental control for live cell imaging.

我们的研究旨在了解质膜信号通路的空间组织和组装动力学如何控制T细胞活化和功能。这项研究是基于我们的发现，质膜蛋白定位在纳米尺寸的域。我们的研究表明，T细胞活化是由不同纳米结构域的空间关系，其中的信号复合物的组装和它们之间的信号分子的易位动态控制的。我们使用多学科的策略，结合联合收割机单分子显微镜（即单粒子跟踪和超分辨率）与生物化学，生物物理学和细胞生物学的方法来研究潜在的分子机制。具体而言，我们感兴趣的是T细胞受体（TCR）和抑制性程序细胞死亡蛋白（PD-1）在T细胞活化和抗肿瘤反应的途径。我们的最终目标是确定T细胞信号通路中的新控制原则，并使用它们来修改T细胞反应，以用于未来的免疫治疗。 我们需要一台先进的全内反射（TIRF）显微镜，这对我们在弗赖堡大学新成立的实验室以及实现我们当前和未来的研究目标至关重要。所要求的显微镜具有以下功能：（i）全内反射显微镜，其中照明仅限于细胞和玻璃表面之间的接触部位。 (ii)用于超分辨率成像和粒子跟踪的单分子检测，以分析蛋白质分布和动力学。图像采集与两个CMOS相机具有高动态范围和大视场。(iii)用于光操作的局部激光控制，以测量蛋白质动力学和分散。多达六个中等功率激光器，覆盖广泛的照明波长范围。(iv)用于快速采集和钙分析的宽视场照明。 (v)活细胞成像的环境控制。