間葉系間質細胞が口腔癌細胞による幹細胞様性質の獲得機構に及ぼす影響についての研究

间充质基质细胞对口腔癌细胞获得干细胞样特性的影响机制研究

• 批准号: 15K20531
• 负责人: 石田 康隆
• 金额: $ 2.58万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2015
• 资助国家: 日本
• 起止时间: 2015-04-01 至 2016-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15K20531/
• 关键词: 口腔癌; 間葉系間質細胞

ヒト間葉系間質細胞が口腔扁平上皮癌細胞の細胞特性に及ぼす影響について検討した。ヒト歯肉由来間葉系間質細胞とヒト舌癌由来扁平上皮癌細胞をトランスウェルを用いて無血清培養系で共培養したところ、ヒト歯肉由来間葉系間質細胞はヒト舌癌由来扁平上皮癌細胞の増殖能を亢進させた。また、ヒト歯肉由来間葉系間質細胞の培養上清は、ヒト舌癌由来扁平上皮癌細胞の細胞遊走能を亢進させた。次に、ヒト舌癌由来口腔扁平上皮癌細胞がヒト歯肉由来間葉系間質細胞の細胞特性に及ぼす影響について検討した。ヒト舌癌由来扁平上皮癌細胞とヒト歯肉由来間葉系間質細胞をトランスウェルを用いて無血清培養系で共培養したが、ヒト舌癌由来扁平上皮癌細胞はヒト歯肉由来間葉系間質細胞の細胞増殖能に影響を及ぼさなかった。しかし、ヒト舌癌由来扁平上皮癌細胞と共培養したヒト歯肉由来間質細胞において腫瘍関連線維芽細胞のマーカーであるFAP (fibroblast activation protein)の発現が亢進していた。

Oral squamous cell carcinoma (OPLC) is characterized by cellular characteristics and cellular characteristics. The origin of tongue cancer is the origin of flat epithelial carcinoma. the cell proliferation of flat epithelial carcinoma can be increased by serum-free culture system. The origin of the meat is that the cell culture supernatant and the flat epithelial carcinoma of the tongue can increase the walking energy of the flat epithelial carcinoma. The origin of squamous cell carcinoma of the tongue, the origin of oral flat epithelial carcinoma, the origin of the flesh, the characteristics of the cells, the characteristics of the cells and the shadow. The origin of the flat epithelial carcinoma of the tongue, the origin of the flesh, the origin of the tumor, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma of the tongue. The origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma, the co-culture of the flat epithelial carcinoma, the origin of the tumor, the origin of the tumor, the origin of the flat epithelial carcinoma of the tongue, the origin of the flat epithelial carcinoma, the flat epithelial carcinoma, the flat epithelial