Recurrent connections between higher olfactory brain areas and their role in mating state-dependent behaviour in Drosophila

果蝇高级嗅觉脑区之间的循环连接及其在交配状态依赖行为中的作用

• 批准号: 506209231
• 负责人: Professorin Dr. Ilona Grunwald Kadow
• 金额: --
• 依托单位: Institut für Physiologie II
• 依托单位国家: 德国
• 项目类别: Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/506209231?language=en
• 关键词: Recurrent; connections; between; higher; olfactory

Internal states strongly influence sensory perception and thereby an animal’s interpretation of the world around it. In project TP3, we focus on a highly important change in internal state, motherhood, and the recurrent circuit mechanisms of how it changes female olfactory perception and behaviour. Female Drosophila melanogaster drastically change behaviour shortly after mating and prioritize finding appropriate egglaying sites rich in nutrients. For finding such sites, females rely on the sense of smell to detect key odorants including the putrid odour of polyamines. Importantly, her preference for polyamines is modulated by mating such that a mated female will prefer higher concentrations of the odour for ~2 weeks after mating. We have previously shown that mating induces changes at several levels in olfactory processing. First, a mating-induced peptidergic signalling modulates polyamine sensing in olfactory sensory neurons. Second, mating also changes higher processing through modulation of specific mushroom body (MB)-innervating dopaminergic neurons (DANs). Most recently, we found that the long-lasting change in mating-induced polyamine perception requires the MB not only for the expression of a changed preference but, surprisingly, also for its mating-related induction. Our latest data suggest that local and long-range recurrent connections between MB output neurons (MBON) and DANs, and MBONs and neurons of the lateral horn (LH) are critical for either induction or expression of higher polyamine preference or both. Here, we will combine behavioural analysis, genetic manipulation, and state-of-the-art in vivo functional imaging with recent connectomics data to map and functionally characterize these recurrent circuit motifs in reproductive state-dependent odour perception in female animals.

内部状态强烈地影响感官知觉，从而影响动物对周围世界的解释。在项目TP3中，我们专注于内部状态、母亲身份的一个非常重要的变化，以及它如何改变女性嗅觉和行为的循环回路机制。雌性黑腹果蝇在交配后不久就会极大地改变行为，优先寻找合适的富含营养的产卵地点。为了找到这样的位置，雌性依靠嗅觉来检测关键的气味，包括多胺的腐烂气味。重要的是，她对多胺的偏好是通过交配来调节的，这样交配后的雌性在交配后的两周内会更喜欢高浓度的气味。我们之前已经证明，交配会在几个层面上引起嗅觉处理的变化。首先，交配诱导的肽能信号调节嗅觉感觉神经元中的多胺感觉。其次，交配还通过调节支配特定蘑菇体(MB)的多巴胺能神经元(DAN)来改变高级加工。最近，我们发现交配诱导的多胺感觉的长期变化不仅需要MB来表达改变的偏好，而且令人惊讶的是，还需要与交配相关的诱导。我们的最新数据表明，MB输出神经元(MBON)和DAN之间以及MBON和侧角神经元(LH)之间的局部和远程重复联系对于诱导或表达更高的多胺偏好或两者都是关键的。在这里，我们将结合行为分析、遗传操作和最先进的活体功能成像与最新的连接学数据来定位和功能描述雌性动物生殖状态依赖气味感知中的这些循环回路基序。