Designing promoter sequences

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We have developed a computational method of designing DNA base sequences of promoters whose transcriptional activities satisfies a given condition (transcriptional strength in a given cell line). The method has introduced two base substitutions into human CRE promoter, then we have verified that these substitutions successfully increased its transcriptional strength in HEK293T cell line ~2.5 times as high as that of the original promoter. On the other hand, it has become clear that designing promoter sequences is interestingly difficult in the case of existing overlapping TFBSs (transcriptional factor binding sites) such as human IFNB promoter.