5-Laser Multiparameter Spectral Flow-Cytometer

5 激光多参数光谱流式细胞仪

• 批准号: 509546995
• 金额: --
• 依托单位: Christian-Albrechts-Universität zu Kiel
• 依托单位国家: 德国
• 项目类别: Major Research Instrumentation
• 财政年份: 2022
• 资助国家: 德国
• 起止时间: 2021-12-31 至 无数据
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/509546995?language=en
• 关键词: Laser; Multiparameter; Spectral; Flow; Cytometer

The multi-parameter spectral analyser is the next generation of flow cytometers, allowing to record the entire emitted fluorescence spectrum. By mathematical deconvolution of the signal into the individual emission spectra of the fluorochromes used, it is possible to examine a considerably higher number of parameters simultaneously than before. Such a spectral analyser, fully equipped with five lasers, is able to measure up to 40 parameters in parallel. In this way, a large amount of information can be obtained from samples that are, for example, only available in limited cell numbers or need to be differentiated into many different cell populations. Thus, in patient material or material from animal experiments, with the help of such a multi-parameter spectral analyser, the most diverse cell populations within a cell mixture can be characterised in detail, with a previously unattainable density of information. This functionality supports ideally the research foci of the UKSH, precision and inflammation medicine, whereby the high-resolution phenotyping of the most diverse cell populations represents an important basis for precision medicine approaches. As part of the newly established Cytometry Core Facility, it will be possible to provide access to this innovative instrument for a wide range of academic users at UKSH, CAU and beyond, and to actively support them in their work. A comparable device is not yet available at the Kiel site, so the acquisition of this device will have a positive impact on the academic landscape in Kiel and will form a distinguishing feature of the Cytometry Core Facility and thus support its establishment.

多参数光谱分析仪是下一代流式细胞仪，允许记录整个发射的荧光光谱。通过用数学方法将信号反卷积成所使用的荧光染料的单个发射光谱，可以同时检查比以前多得多的参数。这样的光谱分析仪，完全配备了五个激光器，能够并行测量多达40个参数。通过这种方式，可以从样本中获得大量的信息，例如，只有有限的细胞数量或需要分化成许多不同的细胞群体。因此，在患者材料或动物实验材料中，借助这种多参数光谱分析仪，可以详细表征细胞混合物中最多样化的细胞群，具有以前无法获得的信息密度。该功能理想地支持UKSH的研究重点，精确和炎症医学，其中最多样化的细胞群的高分辨率表型代表了精确医学方法的重要基础。作为新建立的细胞术核心设施的一部分，它将有可能为UKSH， CAU和其他地方的广泛学术用户提供这种创新仪器，并积极支持他们的工作。在基尔基地还没有类似的设备，因此该设备的收购将对基尔的学术环境产生积极影响，并将形成细胞术核心设施的一个显著特征，从而支持其建立。