5-Laser Multiparameter Spectral Flow-Cytometer

多参数光谱分析仪是下一代流式细胞仪，允许记录整个发射的荧光光谱。通过将信号数学解卷积成所使用的荧光染料的各个发射光谱，可以同时检查比以前多得多的参数。这种光谱分析仪完全配备了五台激光器，能够并行测量多达40个参数。通过这种方式，可以从样本中获得大量信息，例如，这些样本只能在有限的细胞数量中获得，或者需要区分成许多不同的细胞群体。因此，在患者材料或来自动物实验的材料中，在这样的多参数光谱分析仪的帮助下，细胞混合物中最多样化的细胞群体可以用以前无法获得的信息密度来详细描述。这一功能理想地支持了UKSH、精密和炎症医学的研究重点，从而使最多样化的细胞群体的高分辨率表型成为精确医学方法的重要基础。作为新设立的细胞学核心设施的一部分，将有可能为英国皇家壳牌大学、中国农业大学和其他地方的广泛学术用户提供使用这一创新仪器的机会，并积极支持他们的工作。基尔基地目前还没有类似的设备，因此该设备的收购将对基尔的学术格局产生积极影响，并将形成细胞计数核心设施的一个显著特征，从而支持其建立。

The multi-parameter spectral analyser is the next generation of flow cytometers, allowing to record the entire emitted fluorescence spectrum. By mathematical deconvolution of the signal into the individual emission spectra of the fluorochromes used, it is possible to examine a considerably higher number of parameters simultaneously than before. Such a spectral analyser, fully equipped with five lasers, is able to measure up to 40 parameters in parallel. In this way, a large amount of information can be obtained from samples that are, for example, only available in limited cell numbers or need to be differentiated into many different cell populations. Thus, in patient material or material from animal experiments, with the help of such a multi-parameter spectral analyser, the most diverse cell populations within a cell mixture can be characterised in detail, with a previously unattainable density of information. This functionality supports ideally the research foci of the UKSH, precision and inflammation medicine, whereby the high-resolution phenotyping of the most diverse cell populations represents an important basis for precision medicine approaches. As part of the newly established Cytometry Core Facility, it will be possible to provide access to this innovative instrument for a wide range of academic users at UKSH, CAU and beyond, and to actively support them in their work. A comparable device is not yet available at the Kiel site, so the acquisition of this device will have a positive impact on the academic landscape in Kiel and will form a distinguishing feature of the Cytometry Core Facility and thus support its establishment.