5-Laser Multiparameter Spectral Flow-Cytometer

多参数光谱分析仪是下一代流式细胞仪，可以记录整个发射的荧光光谱。通过将信号数学解卷积为所用荧光染料的单个发射光谱，可以同时检查比以前多得多的参数。这种光谱分析仪配备了五个激光器，能够并行测量多达40个参数。以这种方式，可以从例如仅在有限细胞数量中可用或需要分化成许多不同细胞群的样品中获得大量信息。因此，在患者材料或来自动物实验的材料中，借助于这种多参数光谱分析仪，可以详细地表征细胞混合物中最多样化的细胞群体，具有先前无法达到的信息密度。该功能理想地支持UKSH的研究重点，精确和炎症医学，其中最多样化的细胞群体的高分辨率表型是精确医学方法的重要基础。作为新成立的细胞计数核心设施的一部分，它将有可能为UKSH，CAU及其他学术用户提供使用这种创新仪器的机会，并积极支持他们的工作。基尔工厂尚未提供类似设备，因此收购该设备将对基尔的学术环境产生积极影响，并将形成细胞计数核心设施的特色，从而支持其建立。

The multi-parameter spectral analyser is the next generation of flow cytometers, allowing to record the entire emitted fluorescence spectrum. By mathematical deconvolution of the signal into the individual emission spectra of the fluorochromes used, it is possible to examine a considerably higher number of parameters simultaneously than before. Such a spectral analyser, fully equipped with five lasers, is able to measure up to 40 parameters in parallel. In this way, a large amount of information can be obtained from samples that are, for example, only available in limited cell numbers or need to be differentiated into many different cell populations. Thus, in patient material or material from animal experiments, with the help of such a multi-parameter spectral analyser, the most diverse cell populations within a cell mixture can be characterised in detail, with a previously unattainable density of information. This functionality supports ideally the research foci of the UKSH, precision and inflammation medicine, whereby the high-resolution phenotyping of the most diverse cell populations represents an important basis for precision medicine approaches. As part of the newly established Cytometry Core Facility, it will be possible to provide access to this innovative instrument for a wide range of academic users at UKSH, CAU and beyond, and to actively support them in their work. A comparable device is not yet available at the Kiel site, so the acquisition of this device will have a positive impact on the academic landscape in Kiel and will form a distinguishing feature of the Cytometry Core Facility and thus support its establishment.