Establishment of simultaneous islet cell and pancreas transplantation from a splitting single-donor pancreas

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We have developed a two-layer pancreas preservation method, which supplies oxygen during preservation, and reported efficacy of its use for pancreas and islet cell transplantation. We apply this concept (oxygenation) to the islet isolation process to obtain more islets. We investigated the efficacy of oxygenation during pancreas digestion process (A), low pressure-controlled injection of collagenase (B) and oxygenation on isolated islets during transportation (C). Islet yield and function were improved in protocol A and B. However, there was no advantage in protocol C.