The research of pathogenesis, development's mechanism and treatment of Pars Tensa Cholesteatoma.

软骨鼓室成形术治疗紧张部胆脂瘤可长期预防术后鼓膜回缩，听力改善良好。中耳炎伴积液与pt -胆脂瘤有一定关系。认为上皮下层成纤维细胞参与了胆脂瘤上皮的活性和胆脂瘤的扩展。上皮和上皮下组织的相互作用在胆脂瘤的发生发展中起重要作用。通过动物实验，将兔鼻黏膜上皮细胞片在温度反应培养板上培养，移植到中耳，证实粘膜再生良好。再生的粘膜在组织学和功能上（中耳腔压力测量）基本正常。我们证实Caspase-14（1.170与上皮细胞分化有关）特异性地在胆脂瘤上皮细胞中发育。提示Caspase-14启动子有助于治疗中耳胆脂瘤（持续存在的胆脂瘤上皮消失）。

Cartilage tympanoplasty for Pars tensa(PT) cholesteatoma prevent postoperative retraction ofn tympanic membrane in the long term, and it was found that the hearing improvement was good. There are some relations between Otisis media with effusion and PT-cholesteatoma. It was thought that fibroblasts in subepithelial layer were involved in activity of cholesteatoma epithelium and cholesteatoma extension. The interaction of epithelium and the subepithelial tissue play ley role for development of cholesteatoma. We cultured a rabbit nasal mucosa epithelial cells sheet in temperature-responsive culture plate by animal experiment, and we transplanted it to the middle ear, and good mucosa regenaration was confirmed. Regenerated mucosa shows almost normal conditions with a histologically and functionally(pressure measurement of the middle ear cavity). Weconfirm that Caspase-14 where 1.170 is associated with for the differentiation of epithelial cells specifically develops in cholesteatoma epithelial cells. It is suggested that Caspase-14 promoter helped treatment (disappearance of the persistentcholesteatoma epithelium) of the middle ear cholesteatoma.