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Utilization of viscoelastic substrates to study actin/MTs coordination and subsequent mechanobiological functionalities

利用粘弹性基质研究肌动蛋白/MTs 协调和随后的力学生物学功能

基本信息

批准号：
22KJ3112
负责人：
Abdelaleem Shimaa
金额：
$ 3.08万
依托单位：
National Institute for Materials Science
依托单位国家：
日本
项目类别：
Grant-in-Aid for JSPS Fellows
财政年份：
2023
资助国家：
日本
起止时间：
2023-03-08 至 2024-03-31
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ3112/
关键词：
Mechanobiology Viscoelastic material EMT Actomyosin ECM concentration nanoparticle microtubules AFM

项目摘要

ECM is the source of diversified mechanical and biochemical cues that alters cellular responses. Deciphering this complexity using biomaterials would increase our understanding of these unique cellular behaviors in ailing and health conditions.In addition to the viscoelastic properties as a mechanical cue, I focused on the ECM concentrations as a chemical cue to investigate the MTs and actomyosin crosstalk. In this work, cRGD as a chemical cue causes an alteration in the actin distribution, and this would alter Actin-MTs crosstalk (Preliminary results was presented at a conference).In joint research, the photoactivatable surfaces were used to investigate the effect of gravity vector on collective cell migration and 3D cellular architectures. These allowed the remote induction of cellular migration. The inversion of cells against the gravity vector resulted in the remodeling of the actomyosin cytoskeletal system that alters the 3D cluster architecture and migratory phenotype (Preliminary results were presented at a conference, paper Accepted STAM 2023).In another joint research, the interactions of the cells with gold nanoparticles functionalized with epidermal growth factor variants were studied. The mechanism for the selective cytotoxic activity of a specific GNP-EGF variant was illustrated (Published in Analytical Science 2023).Another research, we investigated the cytoskeletal response to various environmental stimuli using AFM, we measured the cytoskeletal stress distribution under various conditions (Preliminary results were presented at a conference, Paper submitted)

细胞外基质是改变细胞反应的各种机械和生化信号的来源。使用生物材料破译这种复杂性将增加我们对这些独特的细胞行为在疾病和健康条件下的理解。除了作为机械提示的粘弹性特性之外，我还将重点放在细胞外基质浓度作为化学提示来研究MTS和肌动球蛋白的串扰。在这项工作中，cRGD作为一种化学信号导致肌动蛋白分布的改变，这将改变肌动蛋白-MTS串扰(初步结果已在一次会议上公布)。在联合研究中，利用光活化表面来研究重力矢量对集体细胞迁移和3D细胞结构的影响。这使得远程诱导细胞迁移成为可能。细胞与重力矢量的反转导致了肌动蛋白细胞骨架系统的重塑，从而改变了3D簇的结构和迁移表型(初步结果在一次会议上公布，论文接受了STAM 2023)。在另一项联合研究中，研究了具有表皮生长因子变体功能化的金纳米颗粒与细胞的相互作用。在另一项研究中，我们用原子力显微镜研究了细胞骨架对不同环境刺激的反应，测量了不同条件下细胞骨架的应力分布(初步结果已在一次会议上发表，提交了论文)。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

The surface density of cyclic RGD peptide alters the microtubule-actin interplays

环状 RGD 肽的表面密度改变微管-肌动蛋白相互作用

DOI：
发表时间：
2022
期刊：
影响因子：
0
作者：
S. A. Abdellatef ;H. Wang;J. Nakanishi
通讯作者：
J. Nakanishi

Impact of extracellular matrix ligand densities on TGF-β-induced EMT.

细胞外基质配体密度对 TGF-β 诱导的 EMT 的影响。

DOI：
发表时间：
2021
期刊：
影响因子：
0
作者：
SUZUKI KEI K.;DOI KANDAI;MORISHIMA KAORI;YAMAGAWA HIROMI;MORI TAIKI;WATARI YUYA;OKABE KIMIKO;S. A. Abdellatef and Jun Nakanishi,
通讯作者：
S. A. Abdellatef and Jun Nakanishi,

Precise Tuning and Characterization of Viscoelastic Interfaces for the Study of Early Epithelial-Mesenchymal Transition Behaviors