Utilization of viscoelastic substrates to study actin/MTs coordination and subsequent mechanobiological functionalities

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ECM is the source of diversified mechanical and biochemical cues that alters cellular responses. Deciphering this complexity using biomaterials would increase our understanding of these unique cellular behaviors in ailing and health conditions.In addition to the viscoelastic properties as a mechanical cue, I focused on the ECM concentrations as a chemical cue to investigate the MTs and actomyosin crosstalk. In this work, cRGD as a chemical cue causes an alteration in the actin distribution, and this would alter Actin-MTs crosstalk (Preliminary results was presented at a conference).In joint research, the photoactivatable surfaces were used to investigate the effect of gravity vector on collective cell migration and 3D cellular architectures. These allowed the remote induction of cellular migration. The inversion of cells against the gravity vector resulted in the remodeling of the actomyosin cytoskeletal system that alters the 3D cluster architecture and migratory phenotype (Preliminary results were presented at a conference, paper Accepted STAM 2023).In another joint research, the interactions of the cells with gold nanoparticles functionalized with epidermal growth factor variants were studied. The mechanism for the selective cytotoxic activity of a specific GNP-EGF variant was illustrated (Published in Analytical Science 2023).Another research, we investigated the cytoskeletal response to various environmental stimuli using AFM, we measured the cytoskeletal stress distribution under various conditions (Preliminary results were presented at a conference, Paper submitted)