Identification and Characterization of Novel Components Involved in the Export of Ribosomes from the Nucleolus into the Cytoplasm

核糖体从核到细胞质的转运机制尚不清楚。鉴于核糖体极其复杂的生物发生途径，与其他货物(如tRNA)的核出口相比，核糖体的核出口可能更加复杂。利用60S亚基报告基因(Rpl25p-EGFP)，我们分离到了20个温度敏感型核糖体输出(Rix)突变体。通过互补克隆了这些突变株的野生型基因。这揭示了核糖体输出中存在大量具有不同亚细胞定位和功能的成分。在我们未来的研究中，我们将表征这些Rix因子，这将使我们从分子上深入了解核糖体组装和随后的细胞质运输之间的耦合。

The mechanism of ribosomal export from the nucleus to the cytoplasm is not understood. As compared to the nuclear export of other cargoes (e.g. tRNA), nuclear export of ribosomes is likely to be more complicated, in view of an extremely complex biogenesis pathway of ribosomes. Using a 60S subunit reporter (Rpl25p-eGFP), we have isolated 20 temperature-sensitive ribosomal export (rix) mutants. The wild-type genes of these rix mutants have been cloned by complementation. This revealed that a large number of components with distinct subcellular location and function are involved in ribosome export. In our future research, we will characterize these Rix factors, which should give a molecular insight in the coupling between ribosome assembly and subsequent transport to the cytoplasm.