Comparison of Sodium Channel Currents Between Various Excitable Cells by Planar Lipid Bilayer and Patch Clamp Methods

通过平面脂质双层和膜片钳方法比较各种可兴奋细胞之间的钠通道电流

• 批准号: 63570071
• 负责人: UEHARA Akira
• 金额: $ 0.32万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570071/
• 关键词: Planar lipid bilayer; Na channels; Excitable cells; Naチャネル

We have been interested in the differences of exitabilities between various exitable cells such as nerve, skeletal muscle and heart muscle cells. So, the single channel currents of Na channels were characterized to compare between the three type of Na channels by using the lipid planar bilayer and patch clamp techniques. Kinetic parameters in blocking of puffer fish toxin, TTX to Na channels were precisely dicided. Na channel of cardiac mucle was the most resistant to TTX. After denervation, skeletal muscle Na channels became as TTX-resistant as heart Na channels. Na channel protein might exist as an isozyme peculiar to each cell type which is easily able to be transformed to the other kind of isozyme. Effects of Na channel activators, batrachotoxin and veratridine on various Na channels were also examined. Every type of Na channel exhibited similar exitabilities then. Thus, these results might explain well the differences and similarities in excitability between various tissues.

我们一直对神经、骨骼肌和心肌细胞等各种可退出细胞之间的退出性差异感兴趣。因此，利用脂质平面双层和膜片钳技术对Na通道的单通道电流进行了表征，以比较三种类型的Na通道。精确确定了阻断河豚毒素、TTX至Na通道的动力学参数。心肌的Na通道对TTX的抵抗力最强。去神经支配后，骨骼肌 Na 通道变得与心脏 Na 通道一样具有 TTX 抗性。 Na通道蛋白可能以每种细胞类型特有的同工酶的形式存在，并且很容易转化为另一种同工酶。还检查了钠离子通道激活剂、箭毒毒素和藜芦定对各种钠离子通道的影响。当时每种类型的 Na 通道都表现出相似的退出能力。因此，这些结果可以很好地解释不同组织之间兴奋性的差异和相似之处。