Study on regulation mechanisms of SR Ca2+-induced Ca2+ release by functional sub-domain of ryanodine receptor

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The ryanodine receptors （RyR）release Ca2+ from SR in cardiac myocytes to trigger the muscle contraction. Our study aims at clarifying the structure-function relation of the cytoplasmic domain of the RyR molecule with ligand binding sites. We established experimental methods in which wild-type and mutant recombinant RyRs were expressed in HEK293 cells and single channel currents were recorded from the expressed RyR molecules. Identifying works regarding the amino acid regions of binding sites for ligands such as Ca2+, polyamine, calmodulin arenow in progress.