Autotransporter folding: insights advancing recombinant protein production

自动转运蛋白折叠：推进重组蛋白生产的见解

• 批准号: DP160103294
• 负责人: Dr Denisse Leyton
• 金额: $ 24.29万
• 依托单位: The Australian National University
• 依托单位国家: 澳大利亚
• 项目类别: Discovery Projects
• 财政年份: 2016
• 资助国家: 澳大利亚
• 起止时间: 2016-04-01 至 2020-02-02
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/DP160103294
• 关键词: Autotransporter; folding; insights; advancing; recombinant

Imagine a world in which any protein could be produced using a single production platform. This project aims to make this a reality by reengineering autotransporters, a large family of bacterial virulence factors with a modular structure that makes them amenable to rational design. The project plans to examine the structures and folding behaviour of autotransporters and reengineered derivatives fused to target heterologous proteins using biochemical, biophysical, and structural methods. It is expected that this project will provide fundamental insights into factors that dictate autotransporter folding and stability, which may enhance recombinant protein production and drive discovery of strategies to prevent autotransporter-mediated infection.

想象一下，在一个世界中，任何蛋白质都可以使用单一的生产平台生产。该项目旨在通过重新设计autotorporters使其成为现实，autotorporters是一个具有模块化结构的细菌毒力因子大家族，使其易于合理设计。该项目计划使用生物化学，生物物理和结构方法来检查autotorpers和融合到目标异源蛋白的重组衍生物的结构和折叠行为。预计该项目将为决定自身转运蛋白折叠和稳定性的因素提供基本见解，这可能会增强重组蛋白的生产并推动发现预防自身转运蛋白介导的感染的策略。