Identification of semiochemicals in bumblebees and their social parasites

熊蜂及其社会寄生虫中化学信息素的鉴定

• 批准号: 51257686
• 负责人: Professor Dr. Manfred Ayasse
• 金额: --
• 依托单位: Fachbereich Chemie
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2012-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/51257686?language=en
• 关键词: Identification; semiochemicals; bumblebees; their; social

A primary aim of the project is the identification of semiochemicals with a function in the regulation of reproduction in bumblebee hosts and their social parasites. In a comparative approach, we will include a highly host specific parasite and a parasite that uses different host species. We will perform gas chromatography with antennographic detection (GC-EAD) and measure olfactory induced cardiac responses of odour samples of hosts and parasites to detect compounds that may play a major role as semiochemicals. Subsequently, GC-EAD active compounds will be identified by chemical analyses using the combination of gas chromatography and mass spectrometry (GC-MS) and synthesized. Finally, bioassays with callow host workers that are exposed to various synthetic mixtures of GC-EAD active compounds will be performed in order to identify dominance signals of the host queens or parasite females. We will compare the mechanisms, respectively semiochemicals that act in regulation of worker reproduction in both, hosts and parasites of the two investigated host-parasite systems. In a second part of the project we will identify chemical signals involved in host nest-recognition of the host specific and non host-specific Psithyurs species. We will perform Y-tube tests to compare the attractiveness of nest marking signals of various host- and non-host species to parasite females and electrophysiological and chemical analyses (GC-MS) to identify the active compounds. Finally we will test whether imprinting on host colonies plays a role in host nest searching parasite females. A comparative approach may open the chance to detect and trace basic mechanisms of chemical communication involved in bumblebee hosts and cuckoo bumblebees.

该项目的一个主要目的是鉴定在大黄蜂宿主及其社会寄生虫的繁殖调节中具有功能的符号化学物质。在比较的方法中，我们将包括高度宿主特异性的寄生虫和使用不同宿主物种的寄生虫。我们将采用气相色谱和天线检测（GC-EAD），并测量宿主和寄生虫气味样品的嗅觉诱导的心脏反应，以检测可能作为半化学物质起主要作用的化合物。随后，GC-EAD活性化合物将通过气相色谱-质谱联用（GC-MS）化学分析鉴定并合成。最后，将对暴露于各种GC-EAD活性化合物合成混合物中的未成熟寄主工蜂进行生物测定，以确定寄主蜂王或寄主雌性的优势信号。我们将比较两种被研究的宿主-寄生虫系统中分别在宿主和寄生虫中调节工蚁繁殖的符号化学物质的机制。在该项目的第二部分中，我们将识别宿主特异性和非宿主特异性狐猴物种的宿主巢识别所涉及的化学信号。我们将进行y管测试，比较不同寄主和非寄主物种的巢穴标记信号对雌性寄生虫的吸引力，并进行电生理和化学分析（GC-MS），以鉴定活性化合物。最后，我们将测试寄主群体上的印记是否在寄主寻巢中起作用。一种比较的方法可能为探测和追踪大黄蜂宿主和杜鹃大黄蜂之间化学通讯的基本机制提供机会。