Mechanism and biological function of mammalian DNA Poly-ADP-ribosylation (20*)

聚adp核糖基化（PARylation）被认为是一种广泛存在的蛋白质特异性修饰，与DNA损伤反应密切相关。相反，我们发现哺乳动物细胞的DNA也在生理上被聚合。DNA PARylation是一个可逆的酶促过程，特别是发生在单链DNA的腺嘌呤残基上。我们计划揭示基因组中腺嘌呤PARylation发生的位置，并打算利用小鼠胚胎干细胞研究PARP酶对DNA PARylation的生理作用。

Poly-ADP-ribosylation (PARylation) is regarded as a widespread protein-specific modification closely associated with the DNA damage response. In contrast, we show that also DNA of mammalian cells is physiologically PARylated. DNA PARylation is a reversible enzymatic process and occurs, in specific, on adenine residues within single stranded DNA. We plan to reveal where in the genome adenine PARylation happens, and intend to study the physiological role of DNA PARylation by PARP enzymes using mouse embryonic stem cells.