Interplay between intestinal microbiome and multimodal MRI brain biosignatures for prediction of response to anti-TNF therapy in ulcerative colitis

肠道微生物组与多模态 MRI 脑生物特征之间的相互作用预测溃疡性结肠炎抗 TNF 治疗的反应

• 批准号: 516215273
• 负责人: Professor Dr. Raja Atreya
• 金额: --
• 依托单位: Medizinische Klinik 1 - Gastroenterologie, Pneumologie und Endokrinologie
• 依托单位国家: 德国
• 项目类别: Clinical Research Units
• 资助国家: 德国
• 项目状态: 未结题
• 来源: https://gepris.dfg.de/gepris/projekt/516215273?language=en
• 关键词: Interplay; between; intestinal; microbiome; multimodal

Ulcerative colitis (UC) is one of the major entities of inflammatory bowel diseases (IBD), causing lifelong morbidity. Apart from diarrhea and rectal bleeding, abdominal pain and ongoing fatigue may represent a great illness burden. Introduction of anti-TNF therapy has made a major impact on the clinical benefit of the patient. Nevertheless, it is only effective in a subgroup of patients and there is still a discrepancy between objective control of inflammation and subjective central perception of symptoms and patient related outcomes, respectively. Previous work from our group demonstrated that functional magnetic resonance imaging (fMRI) was able to visualize a rapid decreased pain perception in the brain of IBD patients responding to anti-TNF antibody therapy. This was visible in primary nociceptive areas and limbic areas involved in pain experience, emotions, and body sensation, well before marked alleviation of mucosal inflammation can be achieved. These results might explain the prospective improvement of other clinical symptoms and renders those patients to become therapy responders in the long run. It has been shown that the microbiome affects the gut-brain axis and might also induce changes in brain function and behavior. However, changes in the gut microbial composition have not been directly correlated with MRI changes in UC patients so far. We postulate that an intestinal microbiome dependent disease subtype is reflected by a specific multimodal MRI (mMRI) biosignature and that this can be predictive for anti-TNF therapeutic efficacy. Within our project, we aim to better understand the correlation between the brain, gut microbial composition and prevalent disease activity after initiating anti-TNF therapy in UC patients. Based on our proof-of-concept studies, using the ideal infrastructure at Erlangen and supported by machine learning approaches we will therefore characterize the intestinal microbiome composition in UC patients in regard to response to anti-TNF therapy and correlate mMRI brain data with individual clinical parameters to establish patient subgroup differentiation to predict response to anti-TNF therapy. Furthermore, we aim to define gut microbiome specific, MRI biosignatures to delineate individual disease subtypes and predict response to anti-TNF efficacy in UC patients. Overall, defining specific individual subtypes of UC patients based on characterization of their intestinal microbiome composition in conjunction with individual MRI brain bio-signature and relate it to therapeutic response to anti-TNF therapy would not only increase our pathophysiological disease understanding, but could also open new ways for individualized patient stratification and prediction of treatment response.

溃疡性结肠炎（UC）是炎症性肠病（IBD）的主要疾病之一，可导致终身患病。除了腹泻和直肠出血，腹痛和持续的疲劳可能是一个巨大的疾病负担。抗TNF治疗的引入对患者的临床获益产生了重大影响。然而，它仅在一个患者亚组中有效，并且在炎症的客观控制与症状和患者相关结局的主观中枢感知之间仍然存在差异。 我们小组之前的工作表明，功能性磁共振成像（fMRI）能够观察到对抗TNF抗体治疗有反应的IBD患者大脑中疼痛感知的快速下降。这在主要伤害感受区和涉及疼痛体验、情绪和身体感觉的边缘区中是可见的，远早于可以实现粘膜炎症的显著缓解。这些结果可能解释了其他临床症状的预期改善，并使这些患者成为长期治疗反应者。 研究表明，微生物组会影响肠道-大脑轴，也可能引起大脑功能和行为的变化。然而，到目前为止，肠道微生物组成的变化与UC患者的MRI变化没有直接相关性。我们假设肠道微生物组依赖性疾病亚型由特定的多模态MRI（mMRI）生物特征反映，并且这可以预测抗TNF治疗功效。 在我们的项目中，我们的目标是更好地了解UC患者开始抗TNF治疗后大脑，肠道微生物组成和流行疾病活动之间的相关性。基于我们的概念验证研究，使用埃尔兰根的理想基础设施并在机器学习方法的支持下，我们将因此表征UC患者对抗TNF治疗的反应方面的肠道微生物组组成，并将mMRI脑数据与个体临床参数相关联，以建立患者亚组分化，从而预测对抗TNF治疗的反应。此外，我们的目标是定义肠道微生物组特异性的MRI生物特征，以描述个体疾病亚型并预测UC患者对抗TNF疗效的反应。总体而言，基于其肠道微生物组组成的表征结合个体MRI脑生物特征定义UC患者的特定个体亚型，并将其与抗TNF治疗的治疗反应联系起来，不仅可以增加我们对病理生理疾病的理解，还可以为个性化患者分层和预测治疗反应开辟新的途径。