Production of human renin in microorganism and its application to medicine.

1)从人肾素前体的互补序列推断其一级结构。预测的序列由406个氨基酸组成，前片段和前片段分别携带20个和46个氨基酸。2）基于对乙酰化蛋白酶的整体折叠非常相似的假设，构建了人肾素的三维模型。3）从Charon 4A人类基因组文库中分离并鉴定了人肾素基因。该基因跨越约11.7个内切酶，由10个外显子和9个内含子组成，这些外显子和内含子定位在可能是酶的可变表面环的点上。colitrp启动子-操纵子构建表达载体。转化后的E.大肠杆菌细胞表达高水平（总细胞蛋白的30%）的前肾素作为杂合蛋白的一部分。

1) The primary structure of human renin precursor was deduced from its complementary sequence. The predicted sequence consists of 406 amino acids with a pre- and a prosegment carrying 20 and 46 amino acids, respectively.2) A three-dimensional model of human renin has been constructed based on the assumption that the overall folding of the aspartyl proteases is very similar. As a reference, we used penicillopepsin.3) The human renin gene was isolated from a Charon 4A human genomic library and characterized. The gene spans about 11.7 kilobases and consists of 10 exons and 9 introns that map at points that could be variable surface loops of the enzyme.4) Human prorenin was produded under the transcriptional control of the E. coli trp promoter-operator in the expression vector. The transformed E. coli cell expressed high levels (30% of total cell protein) of prorenin as part of a hybrid protein.