IDIOTYPES AND ANTI-IDIOTYPIC ANTIBODIES OF AUTOANTIBODIES IN CONNECTIVE TISSUE DISEASES

独特型是特定免疫球蛋白分子特有的抗原决定因素，作为抗体的遗传标记，参与免疫调节。因此，对自身抗体独特型的研究可能为我们提供有关特异性自身抗体产生机制的有用信息。本研究制备了抗SSB/La自身抗体的多克隆和单克隆抗独特型抗体，通过等电聚焦将抗SSB/La抗体在狭窄的范围内分级，表明这些抗体是由限制性B细胞克隆产生的。抗SSB/La自身抗体的交叉反应独特型很少见，仅在30例抗SSB/La患者中观察到4例。

The idiotypes, antigenic determinants unique to the particular immunoglobulin molecules, serve as genetic markers of antibodies and involves in the immune regulations. Therefore, the studies of idiotypes of autoantibodies may give us useful informations concerning on the mechanisms of specific autoantibody production. In the present study, poly and monoclonal anti-idiotypic antibodies to autoantibodies to the SSB/La antigen were prepared.The anti-SSB/La antibodies were fractionated in the narrow ranges by the isoelectrofocusing indicating the production by the restricted B-cell clones. The cross reactive idiotypes of anti-SSB/La autoantibodies were rare and oberved in only 4 out of 30 patients with anti-SSB/La.The results of the present study indicate that prolonged antigenic stimulation may play an important role in production of this autoantibody.