粘液細菌の子実体形成期に細胞形態変化を司どる遺伝子の同定

粘细菌子实体形成过程中控制细胞形态变化的基因的鉴定

• 批准号: 08760317
• 负责人: 木村 義雄
• 金额: $ 0.64万
• 依托单位: Kagawa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Encouragement of Young Scientists (A)
• 财政年份: 1996
• 资助国家: 日本
• 起止时间: 1996 至 无数据
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-08760317/
• 关键词: 粘液細菌; 形態変化; トランスポゾン変異株

粘液細菌Myxococcus xanthusは、飢餓条件下で社会的な行動をとり多細胞による分化を行う。固体培地上で飢餓条件におかれると細胞は集合し子実体と呼ばれる多細胞構造体を形成する。子実体への分子に伴いM.xanthus細胞は長桿菌から球形の胞子へと細胞形態を変化させる。本研究ではオートリシスを起こす原因ともいわれ、M.xanthusの分化において必須の現象である細胞の形態変化に関与する遺伝子を同定することを目的とした。そこでまずM.xanthus変異株の作製を行った。転移性遺伝子トランスポゾンV(TnV)をP1ファージを介してM.xanthus染色体に挿入させることで、変異株643株を作製し、分化誘導培地において集合体を形成するものの胞子形成に移行せずに桿菌のままで存在している6種の変異株を分離した。これらの変異株の染色体上におけるTnV挿入領域をクローニングし、変異領域の一部の塩基配列の決定しホモログ検索を行った結果、1種の変異株についてCoAデカルボキシラーゼとのホモロジーが確認された。そこでこの遺伝子の全塩基配列を決定しCoAデカルボキシラーゼとのホモロジーを確認した。次にこの遺伝子の発現時期を調べるため野生株より様々な分化時間におけるRNAを抽出し、変異遺伝子をプローブとしてノーザン分析を行った結果、この遺伝子は栄養細胞には発現があまりみられないものの分化開始と共に発現が増加し、細胞形態が大きく変化する約60時間において発現が急激に増加していた。脂肪酸の合成に関わっていると言われるCoAデカルボキシラーゼの働きを調べるため、変異株と野生株の対数増殖期と分化中の脂肪酸組成を分析した結果、野生株において分化中に増加する一部の分岐脂肪酸が変異株において野生株より低い割合で存在していることが確認された。

Under the condition of myxobacteria Myxococcus xanthus and myxobacteria, the behavior of the society is characterized by multi-cell differentiation. Under the conditions of solid culture, the cells are collected and the multi-cellular structure is formed. Progeny molecules are associated with M.xanthus cells, long bacteria, globular cells, cytopathic cells, cytopathic cells. In this study, it is necessary to determine the cause of the disease, and the differentiation of M.xanthus must be the same as that of the cell and the child. Please tell me that the M.xanthus plant is the best one for you. The chromosomes of TnV were transferred into E. coli, 643 strains of E. coli were used as isolates, and the aggregates were formed by differentiation. There were 6 kinds of isolates in the culture of transitional bacteria. In other words, the TnV on the chromosomes of this strain is registered in the field. In the field, a basic array is used to determine the results of the test, and one strain is used to determine the results of the database. The results show that the TnV is registered in the field, and that in the field, the results of the database are determined. The results show that the results of the test, and the results of the test, the results of the test, the results of the data, the results of the data, the results, and the results. The full base column determines that the CoA is not valid. During the current period, the time of differentiation of wild strains of wild strains was detected, and the results of the analysis of the results were analyzed. The results of the analysis of the results, the results of the cell cycle, the beginning of the differentiation of wild strains, the time of differentiation of wild strains and the time of differentiation of wild strains were detected. The size of the cell is very large, and it takes about 60 hours to experience an emergency and increase the number of patients. The results of fatty acid composition analysis during the reproductive period differentiation of the wild strains, the results of the analysis of fatty acid composition in the differentiation of the wild strains, and the presence of a part of the bifurcated fatty acids in the differentiation of the wild strains were confirmed by the analysis of the composition of fatty acids in the differentiation of the wild strains, the results of the analysis of the composition of fatty acids in the reproductive period, the results of the analysis of the composition of fatty acids, the results of the analysis of the composition of fatty acids, the results of the analysis of the composition of fatty acids in the reproductive period, the results of the analysis of fatty acid composition in the differentiation of wild strains, the results of the analysis of fatty acid composition, the results of the analysis of fatty acid composition in the differentiation of wild strains, and the presence of bifurcated fatty acids in the differentiation of wild strains.