Molecular mechanism of daikadaptation regulated by rhodopsin phosphorylation

为了了解视紫红质磷酸化与暗适应的关系，我们研究了几种条件下视紫红质磷酸化的动力学，得到了以下结果：1)在体内，视紫红质磷酸化发生在两个不同的位置，并且磷酸化和去磷酸化的动力学不同。2)视紫红质磷酸化只催化视紫红质激酶而不是蛋白激酶。3)视紫红质C末端的截断改变了磷酸化的分布和动力学。4)位于C端的磷酸化丝氨酸的去磷酸化比位于膜附近的要快。

To understnad the relationship between rhodopsin phosphorylation and dark adaptation, kinetics of rhodopsin phosphorylation was investigated under several conditions and obtained following results.1)In vivo, rhodopsin phosphorylation was occurred at two different sites and the kinetics of phosphorylation and dephosphorylation was different with each others.2)Rhodopsin phosphorylation was catalyzed only rhodopsin kinase not protein kinase C.3)The distribution and kinetics of phosphorylation was modified by the truncation of the C-terminus of rhodopsin.4)The dephosphorylation of the phospho Ser located C-terminus was faster than those located near membrane.