Study on molecular mechanism causing cancer-associated retinopathy by aberrant expression of photoreceptor specific recoverin

为了阐明CAR的分子病理，我们检测了recoverin的表达。在30-70%的癌组织中发现有Recoverin表达。我们从293或SSTW细胞裂解物中观察到g蛋白偶联激酶（GRKs）和小窝蛋白-1与Rec共沉淀。免疫细胞化学显示，肿瘤细胞内对恢复的免疫反应性与对GRKs和caveolin-1的免疫反应性几乎相同。目前的数据强烈提示，异常表达的recoverin可能参与了grk依赖性的癌细胞细胞调节。

T o elucidate the molecular pathology in CAR, expressions of recoverin were examined. Recoverin was identified to be expressed in 30-70% of cancer tissues. We observed co-precipitation of G-protein-coupled-kinases (GRKs) and caveolin-1 with Rec from cell lysates of 293 or SSTW cells. Immunocytechemistry revealed that immunoreactivitiestoward recoverin within the cancer cells were almost identical to those toward GRKs and caveolin-1. The present data strongly suggests that aberrantly expressed recoverin should be involved in the GRK-dependent cellular regulation in cancer cells.