High Resolution of Chiral Compounds Using Protein-Multilayered Porous Membranes

使用蛋白质多层多孔膜高分辨率手性化合物

基本信息

批准号：
09450289
负责人：
SAITO Kyoichi
金额：
$ 3.07万
依托单位：
Chiba University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09450289/
关键词：
Chiral Separation Bovine Serum Albumin Adsorption in Multilayers Porous Membrane Hollow Fiber Radiation-Induced Grafting Permeation Mode High Resolution 中空糸

项目摘要

Chiral separation is an essential technology for producing effective and safe pharmaceuticals. At present, selective crystallization and chiral chromatography are used to resolve various racemic chemicals ; the latter is more versatile in that the chiral ligands can be immobilized on supporting matrices.We describe a bovine serum albumin (BSA)-multilayer-adsorbed porous hollow-fiber membrane as a stationary phase that enables chiral separations at a high resolution and high rate. Epoxy-group-containing graft chains were uniformly immobilized on the surface of pores throughout a porous hollow-fiber membrane by radiation-induced graft polymerization. Subsequently, a diethylamino group as an anion-exchange moiety was introduced to the graft chains, which caused the chains to expand toward the interior of the pores due to mutual electrostatic repulsion. The expanding polymer chains provided multilayer binding sites for BSA as a chiral selector. BSA with a degree of multilayer binding of four specifically recognized L-tryptophan with a separation factor of 6.6 during permeation by a mobile phase (Tris-HCl buffer) injected with a racemic solution of DL-tryptophan through the BSA-multilayered porous membrane. In addition, the separation factor was constant irrespective of flow rates of the mobile phase because of negligible diffusional mass-transfer resistance of tryptophan to BSA multilayered by the graft chains.

手性分离是生产有效且安全的药物的重要技术。目前，选择性结晶和手性色谱法用于解析各种外围化学物质。后者更具用途，因为手性配体可以固定在支撑矩阵上。我们描述了牛血清白蛋白（BSA） - 多型载多孔的空心纤维膜作为固定相，可以以高分辨率和高速速率以手势分离。通过辐射诱导的移植聚合聚合，将含环氧组的移植链均匀地固定在孔的表面上。随后，将二乙基氨基作为一个阴离子交换部分引入移植链，这导致链条由于相互静电排斥而向孔内延伸。扩展的聚合物链为手性选择器提供了BSA的多层结合位点。 BSA具有四个特定识别的L-色氨酸的多层结合度的BSA，分离因子在流动相（TRIS-HCL缓冲液）渗透期间为6.6，并通过BSA-多孔多孔膜注入了DL- tryptophan的外星溶液。另外，由于tonapophan对移植链对BSA的扩散质量转移的耐药性可忽略不计，因此分离因子持续不断。