Highly Efficient Monitoring and Purification of Environments Using Protein-Multilayered Structure in Polymer Brush

通过辐射引发接枝聚合，在多孔中空纤维膜的孔表面接枝了一种含环氧基团的聚合物刷。由于带电基团的静电斥力，聚合物刷的环氧基团转变为各种带电基团，导致聚合物刷的伸展。延伸聚合物刷形成的空间为蛋白质提供了与聚合物刷的三维结合，本研究将改性聚合物刷应用于有害分子和离子的快速检测和纯化。我们成功地使用了一种新型的抗雌激素-抗抗体固定化多孔膜，该多孔膜是通过抗体与接枝在多孔中空纤维膜上的聚合物刷的环氧基反应而制备的，成功地实现了17β-雌二醇的特定浓度。此外，我们还通过疏水相互作用将萃取剂浸渍到含烷基氨基的聚合物刷子上，制备了高性能的中空纤维膜。所制得的中空纤维膜表现出以PdCl4^&lt；2-&gt；为代表的络合离子物种的金属离子的特殊聚集。

An epoxy-group-containing polymer brush was grafted onto the pore surface of a porous hollow-fiber membrane by radiation-induced graft polymerization. The conversion of the epoxy group of the polymer brush into various charged groups resulted in the extension of the polymer brush due to the electrostatic repulsion of the charged group. The space formed by the extended polymer brush provided the proteins with three-dimensional binding to the polymer brush.In this study, the modified polymer brush was applied to a rapid determination and purification of harmful molecules and ions. We succeeded in the specific concentration of 17 β-estradiol using a novel anti-estrogen-anitibody-immobilized porous membrane, which was prepared via a reaction of the antibody with the epoxy group of the polymer brush grafted onto the porous hollow-fiber membrane. Furthermore, we prepared a high-performance porous hollow-fiber membrane impregnating the extractants onto an alkylamino-group-containing polymer brush via hydrophobic interaction. The resultant porous hollow-fiber membrane exhibited a specific collection of the metal ions dissolved as complex ionic species representative of PdCl_4^<2->.